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Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker.
- Source :
-
African health sciences [Afr Health Sci] 2019 Mar; Vol. 19 (1), pp. 1346-1352. - Publication Year :
- 2019
-
Abstract
- Introduction: Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method.<br />Methods: A total of 1521 stool samples were collected from the study individuals. Initially, the identification of hymenolepis nana was confirmed by parasitological method including direct wet-mount and formalin-ethyl acetate concentration methods. Afterward, PCR-sequencing analysis of ribosomal ITS2 fragment was targeted to investigate the molecular identification of the parasite.<br />Results: Overall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration. All ten isolates were succefully amplified by PCR and further sequenced. The determined sequences were deposited in GenBank under the accession numbers MH337810 -MH337819.<br />Conclusion: Our results clarified the presence of H. nana among the patients in the study areas. In addition, the molecular technique could be accessible when the human eggs are the only sources available to identify and diagnose the parasite.
- Subjects :
- Amplified Fragment Length Polymorphism Analysis
Animals
Feces parasitology
Genetic Markers genetics
Humans
Hymenolepiasis diagnosis
Hymenolepis nana isolation & purification
Iran
Phylogeny
Polymerase Chain Reaction
DNA, Ribosomal genetics
Hymenolepiasis parasitology
Hymenolepis nana genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1729-0503
- Volume :
- 19
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- African health sciences
- Publication Type :
- Academic Journal
- Accession number :
- 31148960
- Full Text :
- https://doi.org/10.4314/ahs.v19i1.6