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Eosinophil extracellular trap formation is closely associated with disease severity in chronic rhinosinusitis regardless of nasal polyp status.
- Source :
-
Scientific reports [Sci Rep] 2019 May 30; Vol. 9 (1), pp. 8061. Date of Electronic Publication: 2019 May 30. - Publication Year :
- 2019
-
Abstract
- Chronic rhinosinusitis (CRS) is a heterogeneous inflammatory airway disease involving non-eosinophilic and eosinophilic phenotypes, which translate to various endotypes. Activated eosinophils and neutrophils are known to generate extracellular traps consisting of DNA and cytotoxic granule proteins. We sought to investigate the presence of eosinophil and neutrophil extracellular traps (EETs and NETs, respectively) in human CRS tissues and to clarify the associations with their clinical features. Nasal polyp (NP) or ethmoid tissue slides of 43 subjects from endoscopic sinus surgery for CRS were analysed. Quantitative analysis of EETs and NETs was performed by confocal microscopy using immunofluorescent staining. For correlation study, the presence of NPs, number of infiltrating tissue eosinophils, preoperative Lund-Mackay scores, and other comorbidities were analysed. EET formation was observed to varying degrees in all CRS groups and was correlated with the number of tissue eosinophils (r = 0.83, p < 0.001) regardless of the presence of NPs. Patients with more EETs demonstrated higher Lund-Mackay scores (r = 0.51, p = 0.009), blood eosinophilia (r = 0.80, p < 0.001), and decreased olfactory function (r = -0.65, p < 0.001). No correlation between the extent of EET formation and the presence of atopy or asthma was apparent. However, none of the CRS groups containing neutrophils formed NETs in this study. Eosinophilic CRS indicates the presence of EETs. Formation of EETs could have a role in clinical decision-making and prediction of treatment outcome of CRS, regardless of NP status.
- Subjects :
- Adult
Chronic Disease
Endoscopy
Eosinophils metabolism
Extracellular Traps immunology
Female
Humans
Male
Middle Aged
Nasal Mucosa cytology
Nasal Mucosa diagnostic imaging
Nasal Mucosa immunology
Nasal Mucosa surgery
Nasal Polyps diagnosis
Nasal Polyps epidemiology
Nasal Polyps immunology
Neutrophils immunology
Neutrophils metabolism
Paranasal Sinuses cytology
Paranasal Sinuses diagnostic imaging
Paranasal Sinuses immunology
Paranasal Sinuses surgery
Rhinitis blood
Rhinitis immunology
Rhinitis surgery
Sinusitis blood
Sinusitis immunology
Sinusitis surgery
Tomography, X-Ray Computed
Young Adult
Eosinophils immunology
Extracellular Traps metabolism
Rhinitis diagnosis
Severity of Illness Index
Sinusitis diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 31147604
- Full Text :
- https://doi.org/10.1038/s41598-019-44627-z