Back to Search
Start Over
Ethanol Extract of Centipeda minima Exerts Antioxidant and Neuroprotective Effects via Activation of the Nrf2 Signaling Pathway.
- Source :
-
Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2019 Apr 03; Vol. 2019, pp. 9421037. Date of Electronic Publication: 2019 Apr 03 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Oxidative stress is implicated in the pathogenesis of neurodegeneration and other aging-related diseases. Previous studies have found that the whole herb of Centipeda minima has remarkable antioxidant activities. However, there have been no reports on the neuroprotective effects of C. minima , and the underlying mechanism of its antioxidant properties is unclear. Here, we examined the underlying mechanism of the antioxidant activities of the ethanol extract of C. minima (ECM) both in vivo and in vitro and found that ECM treatment attenuated glutamate and tert-butyl hydroperoxide (tBHP)-induced neuronal death, reactive oxygen species (ROS) production, and mitochondria dysfunction. tBHP-induced phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun N-terminal kinases (JNK) was reduced by ECM, and ECM sustained phosphorylation level of extracellular signal regulated kinase (ERK) in SH-SY5Y and PC12 cells. Moreover, ECM induced the activation of nuclear factor erythroid 2-related factor 2 (Nrf2) and the upregulation of phase II detoxification enzymes, including heme oxygenase-1 (HO-1), superoxide dismutase-2 (SOD2), and NAD(P)H quinone oxidoreductase-1 (NQO-1) in both two cell types. In a D-galactose (D-gal) and aluminum muriate (AlCl <subscript>3</subscript> )-induced neurodegenerative mouse model, administration of ECM improved the learning and memory of mice in the Morris water maze test and ameliorated the effects of neurodegenerative disorders. ECM sustained the expression level of postsynaptic density 95 (PSD95) and synaptophysin (SYN), activated the Nrf2 signaling pathway, and restored the levels of cellular antioxidants in the hippocampus of mice. In addition, four sesquiterpenoids were isolated from C. minima to identify the bioactive components responsible for the antioxidant activity of C. minima ; 6- O -angeloylplenolin and arnicolide D were found to be the active compounds responsible for the activation of the Nrf2 signaling pathway and inhibition of ROS production. Our study examined the mechanism of C. minima and its active components in the amelioration of oxidative stress, which holds the promise for the treatment of neurodegenerative disease.
- Subjects :
- Animals
Antioxidants isolation & purification
Asteraceae chemistry
Cell Death drug effects
Cell Line, Tumor
Ethanol chemistry
Humans
Male
Mice
Mitochondria drug effects
Neurons drug effects
Neurons metabolism
Neuroprotective Agents isolation & purification
Oxidative Stress drug effects
PC12 Cells
Plant Extracts isolation & purification
Plant Extracts pharmacology
Random Allocation
Rats
Reactive Oxygen Species antagonists & inhibitors
Reactive Oxygen Species metabolism
Signal Transduction drug effects
Antioxidants pharmacology
NF-E2-Related Factor 2 metabolism
Neuroprotective Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1942-0994
- Volume :
- 2019
- Database :
- MEDLINE
- Journal :
- Oxidative medicine and cellular longevity
- Publication Type :
- Academic Journal
- Accession number :
- 31139305
- Full Text :
- https://doi.org/10.1155/2019/9421037