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Evaluation of Thin Layer Agar 7H11 for Direct Susceptibility Testing of Mycobacterium Tuberculosis Complex against Second Line Anti Tuberculosis Drugs on Smear Positive Specimens.
- Source :
-
Journal of the College of Physicians and Surgeons--Pakistan : JCPSP [J Coll Physicians Surg Pak] 2019 Jun; Vol. 29 (6), pp. 520-523. - Publication Year :
- 2019
-
Abstract
- Objective: To evaluate performance of thin layer agar (TLA) 7H11 method for detection of ofloxacin (OFX) and kanamycin (KM) resistance in smear positive clinical specimens of patients with tuberculosis comparing the results with gold standard MGIT 960 system.<br />Study Design: Cross-sectional validation study.<br />Place and Duration of Study: Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, from April to September 2017.<br />Methodology: Acid fast bacilli (AFB) smear positive specimens submitted at the study place, were inoculated on TLA 7H11 agar. Growth was examined along with susceptibility of OFX and KM and compared with gold standard MGIT 960 system.<br />Results: One hundred and sixty specimens were evaluated. Sensitivity and specificity of TLA for OFX was found to be 100% and 99.3%, respectively; and PPV and NPV was found to be 90.9% and 100%, respectively. Overall diagnostic accuracy was 99.38%. Sensitivity and specificity of TLA for KM was found to be 80% and 100%, respectively. PPV and NPV was found to be 100% and 99.36%, respectively. Overall diagnostic accuracy was 99.38%.<br />Conclusion: Thin layer agar is reliable, easy to perform and cost effective technique not only for rapid detection of MTB but also for drug susceptibility (DST) of second line anti TB agents. It is a suitable alternative to culture on LJ medium and can also be alternative to MGIT 960 system in resource-poor settings.
- Subjects :
- Adolescent
Adult
Aged
Aged, 80 and over
Antitubercular Agents pharmacology
Chromatography, Thin Layer
Cross-Sectional Studies
Humans
Isoniazid pharmacology
Middle Aged
Mycobacterium tuberculosis genetics
Mycobacterium tuberculosis growth & development
Sensitivity and Specificity
Sputum microbiology
Tuberculosis drug therapy
Tuberculosis microbiology
Young Adult
Anti-Bacterial Agents pharmacology
Bacteriological Techniques methods
Drug Resistance, Multiple, Bacterial genetics
Kanamycin pharmacology
Microbial Sensitivity Tests methods
Mycobacterium tuberculosis drug effects
Mycobacterium tuberculosis isolation & purification
Ofloxacin pharmacology
Tuberculosis diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1681-7168
- Volume :
- 29
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of the College of Physicians and Surgeons--Pakistan : JCPSP
- Publication Type :
- Academic Journal
- Accession number :
- 31133148
- Full Text :
- https://doi.org/10.29271/jcpsp.2019.06.520