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Genome sequencing of Bacillus thuringiensis isolate T414 toxic to pink bollworm (Pectinophora gossypiella Saunders) and its insecticidal genes.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2019 Sep; Vol. 134, pp. 103553. Date of Electronic Publication: 2019 May 24. - Publication Year :
- 2019
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Abstract
- Bacillus thuringiensis is a spore-forming bacterium that is pathogenic towards a range of insect and nematode species and had been widely used as a biopesticide. In this study, we present the morphological, molecular and genetic characteristics of an indigenous Bt isolate T414 which displayed an effective toxicity against Pectinophora gossypiella. Scanning electron microscopy revealed the presence of bipyramidal, spherical and cubic shaped protein crystals in its spore-crystal suspension. SDS-PAGE analysis of its spore-crystal mixture showed the presence of two major protein bands viz.130 and 65 kDa. Whole genome sequencing with MiSeq divulged that it contains a chromosome and many plasmids. The assembled genome finally contained 6493494bp. Automated annotation of this genome draft predicted 6877 coding sequences and 152 RNAs (rRNAs, tRNAs and ncRNAs). NCBI blast analysis showed that assembled genome was distributed in a chromosome and 15 different types of plasmids. Further analysis of draft sequence revealed it harbors parasporal crystal protein genes (cry1Aa, cry1Ab, cry1Ac, cry1IAa, cry2Aa, cry2Ab and cyt1), vegetative insecticidal protein gene (vip3Aa), all plasmid borne and various additional virulence factors such as chitinases, proteases, bacteriocins and hemolysins. From the analysis it is evident that all the Cry, Cyt or Vip toxins are plasmid borne and are present on two types of plasmids named as p414A and p414E in the present study. A cry2A type gene was cloned and sequenced. It was named as cry2Aa21 by Bt nomenclature committee.<br /> (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Bacillus thuringiensis isolation & purification
Bacillus thuringiensis metabolism
Bacillus thuringiensis Toxins
Bacterial Proteins chemistry
Bacterial Proteins isolation & purification
Bacterial Proteins metabolism
Bacterial Toxins chemistry
Base Sequence
Endotoxins metabolism
Hemolysin Proteins metabolism
India
Insecticides
Virulence Factors genetics
Bacillus thuringiensis genetics
Bacterial Proteins genetics
Bacterial Toxins genetics
Endotoxins genetics
Hemolysin Proteins genetics
Lepidoptera drug effects
Whole Genome Sequencing
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 134
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 31129311
- Full Text :
- https://doi.org/10.1016/j.micpath.2019.103553