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FruBPase II and ADP-PFK1 are involved in the modulation of carbon flow in the metabolism of carbohydrates in Methanosarcina acetivorans.
- Source :
-
Archives of biochemistry and biophysics [Arch Biochem Biophys] 2019 Jul 15; Vol. 669, pp. 39-49. Date of Electronic Publication: 2019 May 22. - Publication Year :
- 2019
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Abstract
- To enhance our understanding of the control of archaeal carbon central metabolism, a detailed analysis of the regulation mechanisms of both fructose1,6-bisphosphatase (FruBPase) and ADP-phosphofructokinase-1 (ADP-PFK1) was carried out in the methanogen Methanosarcina acetivorans. No correlations were found among the transcript levels of the MA&#95;1152 and MA&#95;3563 (frubpase type II and pfk1) genes, the FruBPase and ADP-PFK1 activities, and their protein contents. The kinetics of the recombinant FruBPase II and ADP-PFK1 were hyperbolic and showed simple mixed-type inhibition by AMP and ATP, respectively. Under physiological metabolite concentrations, the FruBPase II and ADP-PFK1 activities were strongly modulated by their inhibitors. To assess whether these enzymes were also regulated by a phosphorylation/dephosphorylation process, the recombinant enzymes and cytosolic-enriched fractions were incubated in the presence of commercial protein phosphatase or protein kinase. De-phosphorylation of ADP-PFK1 slightly decreased its activity (i.e. Vmax) and did not change its kinetic parameters and oligomeric state. Thus, the data indicated a predominant metabolic regulation of both FruBPase and ADP-PFK1 activities by adenine nucleotides and suggested high degrees of control on the respective pathway fluxes.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Adenosine Diphosphate metabolism
Adenosine Monophosphate metabolism
Adenosine Triphosphate metabolism
Animals
Archaeal Proteins genetics
Archaeal Proteins isolation & purification
Chickens
Fructose-Bisphosphatase genetics
Fructose-Bisphosphatase isolation & purification
Fructosephosphates metabolism
Genes, Archaeal
Kinetics
Methanosarcina genetics
Phosphofructokinase-1 genetics
Phosphofructokinase-1 isolation & purification
Phosphorylation
Protein Kinase Inhibitors metabolism
Protein Processing, Post-Translational
Archaeal Proteins metabolism
Fructose-Bisphosphatase metabolism
Methanosarcina metabolism
Phosphofructokinase-1 metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0384
- Volume :
- 669
- Database :
- MEDLINE
- Journal :
- Archives of biochemistry and biophysics
- Publication Type :
- Academic Journal
- Accession number :
- 31128085
- Full Text :
- https://doi.org/10.1016/j.abb.2019.05.012