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Transcriptional response of Caenorhabditis elegans when exposed to Shigella flexneri.
- Source :
-
Genomics [Genomics] 2020 Jan; Vol. 112 (1), pp. 774-781. Date of Electronic Publication: 2019 May 21. - Publication Year :
- 2020
-
Abstract
- In recent years, researchers have begun to use Caenorhabditis elegans as a potential animal model to study Shigella pathogenesis. This study aims to further develop this model using RNA-sequencing to understand which pathways/cellular characteristics are affected and potentially cause death in Shigella-exposed worms. We identified 1631 differentially expressed genes in Shigella-exposed worms (6 h exposure). A number of these genes encode proteins involved in fatty-acid β-oxidation (FAO), antioxidant defense and autophagy. The down-regulation of acyl-CoA dehydrogenases would impede FAO, reducing the overall energy to combat Shigella in the worm's intestinal tract. This is potentially coupled with the production of reactive oxygen species (ROS) that may not be fully quenched by antioxidant defense proteins, leading to damaged cellular organelles in the worm's intestinal cells. These cells may undergo autophagy to remove the mounting damage, but may eventually undergo cell death.<br /> (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Antioxidants metabolism
Autophagy genetics
Caenorhabditis elegans metabolism
Caenorhabditis elegans microbiology
Disease Models, Animal
Dysentery, Bacillary metabolism
Fatty Acids metabolism
RNA-Seq
Transcriptome
Caenorhabditis elegans genetics
Dysentery, Bacillary genetics
Shigella flexneri
Subjects
Details
- Language :
- English
- ISSN :
- 1089-8646
- Volume :
- 112
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 31125598
- Full Text :
- https://doi.org/10.1016/j.ygeno.2019.05.016