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Cyclohexane, naphthalene, and diesel fuel increase oxidative stress, CYP153, sodA, and recA gene expression in Rhodococcus erythropolis.
- Source :
-
MicrobiologyOpen [Microbiologyopen] 2019 Sep; Vol. 8 (9), pp. e00855. Date of Electronic Publication: 2019 May 22. - Publication Year :
- 2019
-
Abstract
- In this study, we compared the expression of CYP153, sodA, sodC, and recA genes and ROS generation in hydrocarbon-degrading Rhodococcus erythropolis in the presence of cyclohexane, naphthalene, and diesel fuel. The expression of cytochrome P450, sodA (encoding Fe/Mn superoxide dismutase), recA, and superoxide anion radical generation rate increased after the addition of all studied hydrocarbons. The peak of CYP153, sodA, and recA gene expression was registered in the presence of naphthalene. The same substrate upregulated the Cu/Zn superoxide dismutase gene, sodC. Cyclohexane generated the highest level of superoxide anion radical production. Hydrogen peroxide accumulated in the medium enriched with diesel fuel. Taken together, hydrocarbon biotransformation leads to oxidative stress and upregulation of antioxidant enzymes and CYP153 genes, and increases DNA reparation levels in R. erythropolis cells.<br /> (© 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
- Subjects :
- Bacterial Proteins biosynthesis
Bacterial Proteins genetics
Biotransformation
Cyclohexanes metabolism
Gene Expression Profiling
Naphthalenes metabolism
Reactive Oxygen Species analysis
Cyclohexanes toxicity
Gasoline toxicity
Gene Expression Regulation, Bacterial drug effects
Naphthalenes toxicity
Oxidative Stress
Rhodococcus drug effects
Rhodococcus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2045-8827
- Volume :
- 8
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- MicrobiologyOpen
- Publication Type :
- Academic Journal
- Accession number :
- 31119875
- Full Text :
- https://doi.org/10.1002/mbo3.855