Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing.

Authors :: Ingegnere T
Mariotti FR
Pelosi A
Quintarelli C
De Angelis B
Tumino N
Besi F
Cantoni C
Locatelli F
Vacca P
Moretta L
Source :: Frontiers in immunology [Front Immunol] 2019 Apr 30; Vol. 10, pp. 957. Date of Electronic Publication: 2019 Apr 30 (Print Publication: 2019).
Publication Year :: 2019
Abstract: CAR-NK cells may represent a valuable tool, complementary to CAR-T cells, in adoptive immunotherapy of leukemia and solid tumors. However, gene transfer to human NK cells is a challenging task, particularly with non-virus-based techniques. Here, we describe a new procedure allowing efficient electroporation-based transfection of plasmid DNA, including CAR and CCR7 genes, in resting or cytokine-expanded human NK cell populations and NK-92 cell line. This procedure may offer a suitable platform for a safe and effective use of CAR-NK cells in adoptive immunotherapy of cancer.

Subjects :: Humans
Jurkat Cells
K562 Cells
Plasmids genetics
Plasmids immunology
Adoptive Transfer
Immunity, Cellular genetics
Killer Cells, Natural immunology
Leukemia immunology
Leukemia therapy
Receptors, CCR7 genetics
Receptors, CCR7 immunology
Receptors, Chimeric Antigen genetics
Receptors, Chimeric Antigen immunology
Transfection

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