Back to Search
Start Over
Long-lasting bactericidal activity through selective physical puncture and controlled ions release of polydopamine and silver nanoparticles-loaded TiO 2 nanorods in vitro and in vivo.
- Source :
-
International journal of nanomedicine [Int J Nanomedicine] 2019 Apr 24; Vol. 14, pp. 2903-2914. Date of Electronic Publication: 2019 Apr 24 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Background: Titanium (Ti) implant-associated infection, which is mostly caused by bacterial adhesion and biofilm formation, may result in implant failure and secondary surgery. Thus it is an urgent issue to prevent bacterial infections at the earliest step. Purpose: To develop a novel surface strategy of polydopamine (PDA) and silver (Ag) nanoparticle-loaded TiO <subscript>2</subscript> nanorods (NRDs) coatings on Ti alloy. Materials and methods: Ag-TiO <subscript>2</subscript> @PDA NRDs was fabricated on Ti alloy by hydrothermal synthesis. The antibacterial activity of Ag-TiO <subscript>2</subscript> @PDA NRDs against Escherichia coli and methicillin-resistant Staphylococcus aureus were tested by FE-SEM, Live/Dead staining, zone of inhibition, bacteria counting method and protein leakage analysis in vitro . In addition, an implant infection model was conducted and the samples were tested by X-ray, Micro-CT and histological analysis in vivo . Besides, cell morphology and cytotoxicity of Mouse calvarial cells (MC3T3-E1) were characterized by FE-SEM, immunofluorescence and CCK-8 test in vitro . Results: Our study successfully developed a new surface coating of Ag-TiO <subscript>2</subscript> @PDA NRDs. The selective physical puncture of bacteria and controlled release of Ag+ ions of Ag-TiO <subscript>2</subscript> @PDA NRDs achieved a long-lasting bactericidal ability and anti-biofilm activity with satisfied biocompatibility. Conclusion: This strategy may be promising for clinical applications to reduce the occurrence of infection in the implant surgeries.<br />Competing Interests: There are no potential conflicts of interest for the authors to disclose in this work.
- Subjects :
- Animals
Bacterial Adhesion drug effects
Cell Adhesion drug effects
Cell Death drug effects
Cell Line
Cell Shape drug effects
Delayed-Action Preparations pharmacology
Escherichia coli drug effects
Escherichia coli ultrastructure
Ions
Methicillin-Resistant Staphylococcus aureus drug effects
Methicillin-Resistant Staphylococcus aureus ultrastructure
Mice
Microbial Sensitivity Tests
Nanotubes ultrastructure
Prosthesis-Related Infections microbiology
Prosthesis-Related Infections pathology
Surface Properties
Anti-Bacterial Agents pharmacology
Indoles chemistry
Metal Nanoparticles chemistry
Nanotubes chemistry
Polymers chemistry
Silver pharmacology
Titanium chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1178-2013
- Volume :
- 14
- Database :
- MEDLINE
- Journal :
- International journal of nanomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 31114199
- Full Text :
- https://doi.org/10.2147/IJN.S202625