Volatiles profile of the different parts of Algerian Bupleurum plantagineum Desf. by headspace solid-phase microextraction and hydrodistillation.

The volatiles from a rare and endemic plant Bupleurum plantagineum Desf collected from Béjaia (Algerian National Park) were analyzed using HS-SPME-GC/MS. The main factors affecting this extraction technique, including fibre coating, ionic forces (salt adding), temperature and time of extraction were performed in order to reach a maximum of sensitivity. The best results were obtained by PDMS fiber using the powdered leaves at specific temperatures for 25 min, without salt adding. On the other hand, the volatile composition of leaves, stems and roots were determined with a clear prevalence of sesquiterpene hydrocarbons of 45.66, 31.99 and 70.28% respectively. A qualitative comparison with essential oil from hydrodistillation was also performed showing differences essentially in minor components.