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Efficient chiral synthesis by Saccharomyces cerevisiae spore encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase in organic solvents.
- Source :
-
Microbial cell factories [Microb Cell Fact] 2019 May 20; Vol. 18 (1), pp. 87. Date of Electronic Publication: 2019 May 20. - Publication Year :
- 2019
-
Abstract
- Background: Saccharomyces cerevisiae AN120 osw2∆ spores were used as a host with good resistance to unfavorable environment. This work was undertaken to develop a new yeast spore-encapsulation of Candida parapsilosis Glu228Ser/(S)-carbonyl reductase II and Bacillus sp. YX-1 glucose dehydrogenase for efficient chiral synthesis in organic solvents.<br />Results: The spore microencapsulation of E228S/SCR II and GDH in S. cerevisiae AN120 osw2∆ catalyzed (R)-phenylethanol in a good yield with an excellent enantioselectivity (up to 99%) within 4 h. It presented good resistance and catalytic functions under extreme temperature and pH conditions. The encapsulation produced several chiral products with over 70% yield and over 99% enantioselectivity in ethyl acetate after being recycled for 4-6 times. It increased substrate concentration over threefold and reduced the reaction time two to threefolds compared to the recombinant Escherichia coli containing E228S and glucose dehydrogenase.<br />Conclusions: This work first described sustainable enantioselective synthesis without exogenous cofactors in organic solvents using yeast spore-microencapsulation of coupled alcohol dehydrogenases.
Details
- Language :
- English
- ISSN :
- 1475-2859
- Volume :
- 18
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Microbial cell factories
- Publication Type :
- Academic Journal
- Accession number :
- 31109314
- Full Text :
- https://doi.org/10.1186/s12934-019-1137-6