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Equine placentitis is associated with a downregulation in myometrial progestin signaling†.
- Source :
-
Biology of reproduction [Biol Reprod] 2019 Jul 01; Vol. 101 (1), pp. 162-176. - Publication Year :
- 2019
-
Abstract
- The current study aimed to elucidate the mechanisms underlying myometrial activation during equine placentitis related to progestogens and the progesterone receptor signaling pathways. Placentitis was induced via intracervical inoculation with Streptococcus equi ssp zooepidemicus in mares at approximately 290 days of gestation (placentitis group; n = 6) with uninoculated gestationally matched mares as controls (n = 4). Mares in the placentitis and control groups were euthanized, and myometrial samples were collected from two regions: region 1-parallel to active placentitis lesion with placental separation in placentitis group (P1) or caudal pole of the placenta in control group (C1); and region 2-parallel to apparently normal placenta without separation in placentitis group (P2) or uterine body in control group (C2). In the current study, SRD5A1 and AKR1C23, which encode for the key P4 metabolizing enzymes, were downregulated in P1 in comparison to C1, C2, and P2, and this was associated with a decline (P < 0.05) in 5αDHP, allopregnanolone (3αDHP), and 20αDHP in P1 in comparison to C1. Further, myometrial expression of PR was downregulated (P < 0.05) in P1 in comparison to C1 and P2, and this was associated with activation of the inflammatory cascade as reflected by significant upregulation of IL-1β and IL-8 in P1 in comparison to C1, C2, and P2, and supported by increased tissue leukocytes in P1 in comparison to C1. In conclusion, equine placentitis is associated with a localized withdrawal of progestins and a downregulation of the PR in the myometrium concomitant with upregulation of inflammatory cytokines and subsequent myometrial activation.<br /> (© The Author(s) 2019. Published by Oxford University Press on behalf of Society for the Study of Reproduction.)
- Subjects :
- Animals
Case-Control Studies
Chorioamnionitis genetics
Chorioamnionitis metabolism
Chorioamnionitis pathology
Chorioamnionitis veterinary
Cytokines genetics
Cytokines metabolism
Down-Regulation genetics
Female
Gene Expression Regulation genetics
Horse Diseases genetics
Horse Diseases pathology
Inflammation Mediators metabolism
Myometrium pathology
Placenta Diseases genetics
Placenta Diseases pathology
Placenta Diseases veterinary
Pregnancy
Pregnancy Complications, Infectious genetics
Pregnancy Complications, Infectious metabolism
Pregnancy Complications, Infectious pathology
Pregnancy Complications, Infectious veterinary
Progestins genetics
Receptors, Steroid genetics
Receptors, Steroid metabolism
Signal Transduction genetics
Horse Diseases metabolism
Horses genetics
Horses metabolism
Myometrium metabolism
Placenta Diseases metabolism
Progestins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1529-7268
- Volume :
- 101
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biology of reproduction
- Publication Type :
- Academic Journal
- Accession number :
- 31107530
- Full Text :
- https://doi.org/10.1093/biolre/ioz059