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Development and validation of RP-HPLC method for simultaneous determination of cefpodoxime proxetil and H2 receptor antagonists in pharmaceutical dosage forms.
- Source :
-
Pakistan journal of pharmaceutical sciences [Pak J Pharm Sci] 2019 Mar; Vol. 32 (2 (Supplementary)), pp. 839-844. - Publication Year :
- 2019
-
Abstract
- A new method on RP-HPLC is devised and validated, as per ICH guidelines, for the synchronous estimation of cefpodoxime proxetil and H2-receptor antagonits that are Cimetidine, Famotidine and Ranitidine. The method is simple, accurate, expeditious, reproducible, robust and precise. Chromatography was done on a C <subscript>18</subscript> (250 x 4.6mm) column with methanol: water as mobile phae in the ratio of 70:30 (v/v), pumped at a flow rate of 1ml/min and pH was maintained using 85% ortho-phosphoric acid at 3. The λ max 240 nm was preferred for UV detection. A good linear relationship was attained, over the concentration ranges of 20-70 μg/ml and 5-30μg/ml, for cefpodoxime proxetil and H <subscript>2</subscript> blockers respectively, with a correlation coefficient of R= 0.9987 to 0.9992. The method was validated and found precised (i.e. intra day and interday analysis) with RSD <2%. LOD and LOQ observations were under 0.4806 to 2.6069μg/ml which proved the method to be sensitive. The method provided satisfactory results of robustness and reproducibility, when validated and applied successfully for analysis of dosage forms.
- Subjects :
- Ceftizoxime analysis
Chromatography, Reverse-Phase methods
Cimetidine analysis
Dosage Forms
Famotidine analysis
Limit of Detection
Ranitidine analysis
Tablets analysis
Cefpodoxime Proxetil
Ceftizoxime analogs & derivatives
Chromatography, High Pressure Liquid methods
Histamine H2 Antagonists analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1011-601X
- Volume :
- 32
- Issue :
- 2 (Supplementary)
- Database :
- MEDLINE
- Journal :
- Pakistan journal of pharmaceutical sciences
- Publication Type :
- Academic Journal
- Accession number :
- 31103980