Back to Search
Start Over
Leucocyte integrins, but neither caspases nor NLR inflammasome are associated with lipopolysaccharide recognition and response in barramundi (Lates calcarifer).
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2019 Aug; Vol. 91, pp. 172-179. Date of Electronic Publication: 2019 May 16. - Publication Year :
- 2019
-
Abstract
- The inflammatory response of fish to LPS is subdued, attributed to absence of TLR4, a key pro-inflammatory receptor for LPS in mammals. Nevertheless, LPS is processed in fish in a T-independent manner and is a protective antigen in fish vaccines, yet pathways for processing LPS in fish remain to be elucidated. Here, we report that caspases and NOD-like receptor inflammasomes typically responsible for LPS recognition and processing in mammals lack critical domains or are absent in barramundi (Lates calcarifer). On the other hand, leucocyte integrins MAC-1 and LFA-1 were detected on the surface of neutrophil- and lymphocyte-like cells respectively in the barramundi spleen by immunocytochemistry, and leucocytes displaying MAC-1 or LFA-1 bound to Factor X and ESM-1 respectively. Exposure to MAC-1 and LFA-1 induced significant IL-1β expression post-stimulation with LPS compared to unstimulated and isotype controls, but the differences observed in TNF-α expression were inconclusive. Our findings implicate MAC-1 and LFA-1 involvement in immune processing of LPS in barramundi and in antigen processing in fish.<br /> (Crown Copyright © 2019. Published by Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 91
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 31103555
- Full Text :
- https://doi.org/10.1016/j.fsi.2019.05.015