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An essential contractile ring protein controls cell division in Plasmodium falciparum.
- Source :
-
Nature communications [Nat Commun] 2019 May 16; Vol. 10 (1), pp. 2181. Date of Electronic Publication: 2019 May 16. - Publication Year :
- 2019
-
Abstract
- During the blood stage of human malaria, Plasmodium falciparum parasites divide by schizogony-a process wherein components for several daughter cells are produced within a common cytoplasm and then segmentation, a synchronized cytokinesis, produces individual invasive daughters. The basal complex is hypothesized to be required for segmentation, acting as a contractile ring to establish daughter cell boundaries. Here we identify an essential component of the basal complex which we name PfCINCH. Using three-dimensional reconstructions of parasites at electron microscopy resolution, we show that while parasite organelles form and divide normally, PfCINCH-deficient parasites develop inviable conjoined daughters that contain components for multiple cells. Through biochemical evaluation of the PfCINCH-containing complex, we discover multiple previously undescribed basal complex proteins. Therefore, this work provides genetic evidence that the basal complex is required for precise segmentation and lays the groundwork for a mechanistic understanding of how the parasite contractile ring drives cell division.
- Subjects :
- Animals
Erythrocytes parasitology
Intravital Microscopy methods
Luminescent Proteins chemistry
Luminescent Proteins genetics
Microscopy, Electron, Transmission
Plasmodium falciparum ultrastructure
Schizonts physiology
Time-Lapse Imaging
Red Fluorescent Protein
Cell Division physiology
Contractile Proteins physiology
Plasmodium falciparum physiology
Protozoan Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 31097714
- Full Text :
- https://doi.org/10.1038/s41467-019-10214-z