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An automated smear microscopy system to diagnose tuberculosis in a high-burden setting.
- Source :
-
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [Clin Microbiol Infect] 2019 Dec; Vol. 25 (12), pp. 1553-1559. Date of Electronic Publication: 2019 May 13. - Publication Year :
- 2019
-
Abstract
- Objectives: TB-EASM (Howsome, Shanghai, China), an automated system combining smear preparation, staining and microscopy in a single platform, was evaluated for tuberculosis (TB) diagnosis in a high disease-burden setting.<br />Methods: Sputum samples from individuals with pulmonary TB were processed in parallel using conventional manual smear microscopy (MS), TB-EASM, liquid culture and GeneXpert. Method sensitivity and specificity were compared with Mycobacterium tuberculosis detection by mycobacteria growth indicator tube (MGIT) and/or GeneXpert MTB/RIF.<br />Results: Of 524 samples, 496 met evaluation criteria for study inclusion. The proportion of M. tuberculosis detected by TB-EASM was 28.2% (150/496), significantly higher than for MS (111/496, 21.2%, p 0.01) and comparable to the rate for MGIT (163/496, 32.9%, p > 0.05). For 190 M. tuberculosis-positive cases identified using MGIT and/or GeneXpert MTB/RIF, the reference standard detection methods, TB-EASM detected 140 positive cases, for an overall sensitivity rate of 73.7% (140/190, 95% CI 67.4-79.9), which was significantly higher than for MS (105/190, 55.3%, 95% CI 48.2-62.3, p < 0.01). Specificities were 96.7% (296/306, 95% CI 94.7-98.7) for TB-EASM and 98.0% (300/306, 95% CI 96.5-99.6) for MS.<br />Conclusion: TB-EASM outperformed conventional MS for M. tuberculosis detection in sputum specimens.<br /> (Copyright © 2019 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Adult
Aged
Automation, Laboratory
Bacteriological Techniques instrumentation
Bacteriological Techniques standards
China epidemiology
Diagnostic Tests, Routine instrumentation
Diagnostic Tests, Routine standards
Female
Humans
Male
Microscopy
Middle Aged
Mycobacterium tuberculosis cytology
Sensitivity and Specificity
Sputum microbiology
Tuberculosis, Pulmonary epidemiology
Young Adult
Bacteriological Techniques methods
Diagnostic Tests, Routine methods
Mycobacterium tuberculosis isolation & purification
Tuberculosis, Pulmonary diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1469-0691
- Volume :
- 25
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 31096045
- Full Text :
- https://doi.org/10.1016/j.cmi.2019.04.033