Back to Search
Start Over
Visualization of Intra-neuronal Motor Protein Transport through Upconversion Microscopy.
- Source :
-
Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2019 Jul 01; Vol. 58 (27), pp. 9262-9268. Date of Electronic Publication: 2019 May 28. - Publication Year :
- 2019
-
Abstract
- Cargo transport along axons, a physiological process mediated by motor proteins, is essential for neuronal function and survival. A current limitation in the study of axonal transport is the lack of a robust imaging technique with a high spatiotemporal resolution to visualize and quantify the movement of motor proteins in real-time and in different depth planes. Herein, we present a dynamic imaging technique that fully exploits the characteristics of upconversion nanoparticles. This technique can be used as a microscopic probe for the quantitative in situ tracking of retrograde transport neurons with single-particle resolution in multilayered cultures. This study may provide a powerful tool to reveal dynamic neuronal activity and intra-axonal transport function as well as any associated neurodegenerative diseases resulting from mutation or impairment in the axonal transport machinery.<br /> (© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Subjects :
- Animals
Axons chemistry
Axons metabolism
Brain metabolism
Cells, Cultured
Cellular Reprogramming
Dyneins metabolism
Fibroblasts cytology
Fibroblasts metabolism
Humans
Induced Pluripotent Stem Cells cytology
Infrared Rays
Mice
Microscopy, Fluorescence
Neurons cytology
Protein Transport
Rats
Metal Nanoparticles chemistry
Molecular Motor Proteins metabolism
Neurons metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1521-3773
- Volume :
- 58
- Issue :
- 27
- Database :
- MEDLINE
- Journal :
- Angewandte Chemie (International ed. in English)
- Publication Type :
- Academic Journal
- Accession number :
- 31087740
- Full Text :
- https://doi.org/10.1002/anie.201904208