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Vitamin D improves endothelial barrier integrity and counteracts inflammatory effects on endothelial progenitor cells.
- Source :
-
FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2019 Aug; Vol. 33 (8), pp. 9142-9153. Date of Electronic Publication: 2019 May 14. - Publication Year :
- 2019
-
Abstract
- Endothelial colony-forming cells (ECFCs), a proliferative subpopulation of endothelial progenitor cells, are involved in angiogenesis and endothelial repair. In this study, we investigated endothelial barrier characteristics of ECFCs, whether vitamin D supports cell-cell adhesion and barrier integrity, and how it affects ECFC mobilization and actin dynamics. Although ECFC barrier was disrupted under inflammatory conditions, this effect was rescued by vitamin D treatment, leading to higher stability of an ECFC monolayer. Furthermore, vitamin D enhanced ECFC mobilization toward directional migration. In addition, immunocytochemistry, quantitative real-time PCR, and immunoblotting analysis showed that vitamin D increased endothelial interconnections through vascular endothelial cadherin (VE-cadherin) junctions and by impacting cell dynamics through cofilin and VE-cadherin phosphorylation. Our results suggest that vitamin D treatment efficiently counteracts inflammation in an ECFC monolayer, resulting in higher ECFC barrier integrity. This study provides evidence of a new beneficial effect of vitamin D for ECFC homeostasis.-Schröder-Heurich, B., von Hardenberg, S., Brodowski, L., Kipke, B., Meyer, N., Borns, K., von Kaisenberg, C. S., Brinkmann, H., Claus, P., von Versen-Höynck, F. Vitamin D improves endothelial barrier integrity and counteracts inflammatory effects on endothelial progenitor cells.
- Subjects :
- Adherens Junctions drug effects
Antigens, CD genetics
Antigens, CD physiology
Cadherins genetics
Cadherins physiology
Capillary Permeability drug effects
Capillary Permeability physiology
Cell Adhesion drug effects
Cell Adhesion physiology
Cell Movement drug effects
Cell Movement physiology
Cells, Cultured
Coculture Techniques
Colony-Forming Units Assay
Endothelial Progenitor Cells pathology
Human Umbilical Vein Endothelial Cells
Humans
Inflammation pathology
Inflammation physiopathology
Tumor Necrosis Factor-alpha metabolism
Vitamin D physiology
Endothelial Progenitor Cells drug effects
Endothelial Progenitor Cells physiology
Inflammation drug therapy
Vitamin D pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1530-6860
- Volume :
- 33
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology
- Publication Type :
- Academic Journal
- Accession number :
- 31084577
- Full Text :
- https://doi.org/10.1096/fj.201802750RR