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New approach to investigate Common Variable Immunodeficiency patients using spectrochemical analysis of blood.
- Source :
-
Scientific reports [Sci Rep] 2019 May 10; Vol. 9 (1), pp. 7239. Date of Electronic Publication: 2019 May 10. - Publication Year :
- 2019
-
Abstract
- Common variable immune deficiency (CVID) is a primary immunodeficiency disease, characterized by hypogammaglobulinemia, recurrent infections and various complications. The clinical heterogeneity of CVID has hindered identification of an underlying immune defect; diagnosis relies on clinical judgement, alongside evidence-based criteria. The lack of pathognomonic clinical or laboratory features leads to average diagnostic delays of 5 years or more from the onset. Vibrational spectroscopic techniques such as Fourier-transform infrared (FTIR) spectroscopy have recently gained increasing clinical importance, being rapid-, non-invasive and inexpensive methods to obtain information on the content of biological samples. This has led us to apply FTIR spectroscopy to the investigation of blood samples from a cohort of CVID patients; revealing spectral features capable of stratifying CVID patients from healthy controls with sensitivities and specificities of 97% and 93%, respectively for serum, and 94% and 95%, respectively for plasma. Furthermore we identified several discriminating spectral biomarkers; wavenumbers in regions indicative of nucleic acids (984 cm <superscript>-1</superscript> , 1053 cm <superscript>-1</superscript> , 1084 cm <superscript>-1</superscript> , 1115 cm <superscript>-1</superscript> , 1528 cm <superscript>-1</superscript> , 1639 cm <superscript>-1</superscript> ), and a collagen-associated biomarker (1528 cm <superscript>-1</superscript> ), which may represent future candidate biomarkers and provide new knowledge on the aetiology of CVID. This proof-of-concept study provides a basis for developing a novel diagnostic tool for CVID.
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 31076587
- Full Text :
- https://doi.org/10.1038/s41598-019-43196-5