Back to Search
Start Over
Molecular Detection of Class 1 Integron-Associated Gene Cassettes in KPC-2-Producing Klebsiella pneumoniae Clones by Whole-Genome Sequencing.
- Source :
-
Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2019 Oct; Vol. 25 (8), pp. 1127-1131. Date of Electronic Publication: 2019 May 10. - Publication Year :
- 2019
-
Abstract
- The dissemination of antimicrobial resistance genes and the bacterium that harbor them have increasingly become a public concern, especially in low- and middle-income countries. The present study used whole-genome sequencing to analyze 10 KPC-2-producing Klebsiella pneumoniae isolates obtained from clinical specimens originated from Brazilian hospitals. The study documents a relevant "snapshot" of the presence of class 1 integrons in 90% of the strains presenting different gene cassettes ( dfrA30, dfrA15, dfrA12, dfrA14, aadA1, aadA2, and aac(6')Iq ), associated or not with transposons. Two strains presented nonclassical integron (lacking the normal 3'conserved segment). In general, most strains showed a complex resistome, characterizing them as highly resistant. Integrons, a genetically stable and efficient system, confer to bacteria as highly adaptive and low cost evolution potential to bacteria, even more serious when associated with high-risk clones, indicating an urgent need for control and prevention strategies to avoid the spread of resistance determinants in Brazil. Despite this, although the class 1 integron identified in the KPC-2-producing K. pneumoniae clones is important, our findings suggest that other elements probably have a greater impact on the spread of antimicrobial resistance, since many of these important genes were not related to this cassette.
Details
- Language :
- English
- ISSN :
- 1931-8448
- Volume :
- 25
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Microbial drug resistance (Larchmont, N.Y.)
- Publication Type :
- Academic Journal
- Accession number :
- 31074706
- Full Text :
- https://doi.org/10.1089/mdr.2018.0437