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Functions of mountain pine beetle cytochromes P450 CYP6DJ1, CYP6BW1 and CYP6BW3 in the oxidation of pine monoterpenes and diterpene resin acids.
- Source :
-
PloS one [PLoS One] 2019 May 09; Vol. 14 (5), pp. e0216753. Date of Electronic Publication: 2019 May 09 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- The mountain pine beetle (MPB; Dendroctonus ponderosae) is a forest insect pest that attacks several different pine (Pinus) species in its native range of distribution in western North America. MPB are exposed for most of their life cycle to the chemical defenses of their hosts. These defenses are dominated by oleoresin secretions containing mostly various monoterpenes and diterpene resin acids (DRAs). Cytochrome P450 enzymes (P450s) of the MPB are thought to be involved in the metabolism of at least some of these defense compounds. Here we describe the cloning and characterization of three MPB P450s, CYP6DJ1, CYP6BW1 and CYP6BW3, and their functions in the oxidation of various monoterpenes and diterpene resin acids. CYP6DJ1 oxidizes the monoterpenes (+)-(4R)-limonene, (-)-(4S)-limonene and terpinolene and produces (4R,8R)-limonene-8,9-epoxide, (4R,8S)-limonene-8,9-epoxide, (4S,8S)-limonene-8,9-epoxide, (4S,8R)-limonene-8,9-epoxide, perilla alcohol and several unidentified oxidized compounds. These products of CYP6DJ1 were also identified in extracts of MPB treated with the same monoterpenes. CYP6BW1 and CYP6BW3 both oxidize the DRAs abietic acid, dehydroabietic acid, neoabietic acid, levopimaric acid, palustric acid, and isopimaric acid, producing hydroxylated and epoxidized DRAs. CYP6DJ1, CYP6BW1 and CYP6BW3 appear to contribute to the metabolism of oleoresin terpenes as part of the MPB's ability to cope with host defenses.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Animals
Coleoptera genetics
Cyclohexane Monoterpenes metabolism
Cytochrome P450 Family 6 genetics
Diterpenes metabolism
Forests
Insect Proteins genetics
Limonene metabolism
Monoterpenes metabolism
Oxidation-Reduction
Recombinant Proteins genetics
Recombinant Proteins metabolism
Resins, Plant metabolism
Substrate Specificity
Coleoptera metabolism
Coleoptera pathogenicity
Cytochrome P450 Family 6 metabolism
Insect Proteins metabolism
Pinus parasitology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 14
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 31071168
- Full Text :
- https://doi.org/10.1371/journal.pone.0216753