Identifying Toxicant-Interacting Genes Using Forward Genetic Screening in Zebrafish.

Authors :: Hill JT
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1965, pp. 251-259.
Publication Year :: 2019
Abstract: Forward genetic screening is an extremely powerful method for identifying novel genes driving a broad range of phenotypes. This protocol describes the complete process for conducting a forward genetic screen in zebrafish, including mutagenesis with N-ethyl-N-nitrosourea (ENU), mating, phenotypic screening, and genetic mapping.

Subjects :: Animals
Chromosome Mapping
Gene Expression Profiling methods
Gene Expression Regulation drug effects
Mutagenesis
Mutagens
Phenotype
Sequence Analysis, RNA
Ethylnitrosourea toxicity
Genetic Testing methods
Zebrafish genetics
Zebrafish Proteins genetics

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