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Exogenous oestradiol benzoate induces male mice azoospermia through modulation of oxidative stress and testicular metabolic cooperation.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2019 Jun; Vol. 19 (6), pp. 4955-4963. Date of Electronic Publication: 2019 Apr 16. - Publication Year :
- 2019
-
Abstract
- In most cases, exogenous oestradiol benzoate (EB) inhibits spermatogenesis, however, the mechanism underlying this process has not been fully elucidated. The present study investigated the effect of EB on redox equilibrium and glycometabolism in mouse testes. Male Kunming mice were divided into 3 groups and injected with 0, 5 and 10 mg/kg EB, respectively. Histological analysis revealed no sperm and far fewer spermatogenic cells in the testes of EB‑treated mice. Additionally, transmission electron microscopy revealed that mitochondria in Sertoli cells were transformed to vacuoles with irregular cristae in the EB‑treated group. EB also significantly decreased the activities and mRNA expression of catalase, superoxide dismutase, and glutathione peroxidase and increased the activity of nitric oxide synthase and nitric oxide concentration in the testes compared with the control. These results indicated that oxidative damage was caused by EB treatment. With regard to glycometabolism, ATP content and activities of hexokinase and pyruvate kinase were significantly reduced in the EB‑treated group. Although glucose and pyruvate concentrations were significantly increased by EB treatment, levels of lactate, the main energy source of spermatogenic cells, were unchanged. Monocarboxylate transporter 2 (MCT2) and MCT4, which are responsible for lactate transportation, were downregulated by EB. In conclusion, the results of the present study indicated that azoospermia induced by EB in male mice was associated with oxidative damage and the disorder of testicular metabolic cooperation.
- Subjects :
- AMP-Activated Protein Kinases metabolism
Animals
Azoospermia chemically induced
Azoospermia veterinary
Chromatography, High Pressure Liquid
Down-Regulation drug effects
Estradiol pharmacology
Glucose Transporter Type 3 genetics
Glucose Transporter Type 3 metabolism
Hexokinase genetics
Hexokinase metabolism
Male
Mice
Microscopy, Electron
Monocarboxylic Acid Transporters genetics
Monocarboxylic Acid Transporters metabolism
Muscle Proteins genetics
Muscle Proteins metabolism
Phosphorylation drug effects
Sertoli Cells ultrastructure
Spermatogenesis drug effects
Superoxide Dismutase metabolism
Testis metabolism
Testis ultrastructure
Azoospermia pathology
Estradiol analogs & derivatives
Metabolome drug effects
Oxidative Stress drug effects
Testis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 19
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 31059031
- Full Text :
- https://doi.org/10.3892/mmr.2019.10169