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Avenanthramide C from germinated oats exhibits anti-allergic inflammatory effects in mast cells.
- Source :
-
Scientific reports [Sci Rep] 2019 May 03; Vol. 9 (1), pp. 6884. Date of Electronic Publication: 2019 May 03. - Publication Year :
- 2019
-
Abstract
- Mast cells play a crucial role in allergic diseases via the release of inflammatory mediators, particularly histamine and pro-inflammatory cytokines. Avenanthramide (Avn) C, a polyphenol found mainly in oats, is known to exhibit various biological properties. In this study, we aimed to evaluate the effectiveness of Avn C from germinated oats against mast cell-mediated allergic inflammation. For the in vitro study, RBL-2H3, mouse bone marrow-derived mast cells and rat peritoneal mast cells were used. Avn C (1-100 nM) inhibited the immunoglobulin (Ig)E-stimulated mast cells degranulation by suppressing phosphorylation of phosphoinositide 3-kinase and phospholipase Cγ1 and decreasing intracellular calcium levels. It inhibited IgE-stimulated secretion of inflammatory cytokines via suppression of FcεRI-mediated signaling proteins Lyn, Syk, Akt, and nuclear factor-κB. To verify the effects of Avn C in vivo, ovalbumin-induced active systemic anaphylaxis (ASA) and IgE-mediated passive cutaneous anaphylaxis (PCA) models were used. Oral administration of Avn C dose-dependently attenuated the ASA reactions, as evidenced by the inhibition of hypothermia and reduction of elevated serum histamine, IgE, and interleukin-4 levels. Avn C also inhibited the PCA reactions, such as ear swelling and plasma extravasation. Our results suggested that Avn C from germinated oats might be a possible therapeutic candidate for mast cell-mediated allergic inflammation.
- Subjects :
- Animals
Cell Degranulation drug effects
Cytokines metabolism
Male
Mast Cells immunology
Mice
Mice, Inbred ICR
Anti-Allergic Agents pharmacology
Anti-Inflammatory Agents pharmacology
Avena chemistry
Avena growth & development
Germination
Mast Cells drug effects
ortho-Aminobenzoates pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 31053741
- Full Text :
- https://doi.org/10.1038/s41598-019-43412-2