Abnormal sympathetic activity after myocardial ischemia involving P2X4 in dorsal root ganglia.

Authors :: Gong Y
Zhou Y
Yang J
Li S
Wang Z
Rao J
Li L
Yuan H
Shi L
Yang R
Xu X
Liu S
Liang S
Zou L
Source :: Brain research bulletin [Brain Res Bull] 2019 Jul; Vol. 149, pp. 216-221. Date of Electronic Publication: 2019 Apr 30.
Publication Year :: 2019
Abstract: The satellite glial cells (SGCs) of the dorsal root ganglia (DRG) expressed P2X4 receptor. In this study, we investigated the abnormal sympathetic activity after myocardial ischemia (MI) involving P2X4 receptor in the cervical DRG SGC. The results showed that MI injury upregulated the P2X4 receptor mRNA and protein in DRG, and the upregulated P2X4 receptor was co-localized with glial fibrillary acidic protein (GFAP) in DRG SGCs. P2X4 short hairpin RNA (shRNA) treatment decreased the expression of P2X4 receptor, counteracted the upregulation of GFAP and IL-1β and inhibited P38MAPK phosphorylation in DRG of MI rats. These results indicate that application of P2X4 shRNA may reduce P2X4-mediated nociceptive signal via inhibiting DRG afferents to alleviate the abnormal sympathetic activity induced by MI.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)

Subjects :: Animals
Diabetes Mellitus, Experimental metabolism
Diabetic Neuropathies metabolism
Female
Glial Fibrillary Acidic Protein metabolism
Male
Myocardial Ischemia metabolism
Neuralgia metabolism
Purinergic P2X Receptor Antagonists pharmacology
RNA, Small Interfering metabolism
Rats
Rats, Sprague-Dawley
Satellite Cells, Perineuronal metabolism
p38 Mitogen-Activated Protein Kinases metabolism
Ganglia, Spinal metabolism
Ganglia, Spinal physiopathology
Myocardial Ischemia physiopathology
Neuroglia metabolism
Receptors, Purinergic P2X4 metabolism

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