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The effects of mechanical stretch on the biological characteristics of human adipose-derived stem cells.
- Source :
-
Journal of cellular and molecular medicine [J Cell Mol Med] 2019 Jun; Vol. 23 (6), pp. 4244-4255. Date of Electronic Publication: 2019 Apr 24. - Publication Year :
- 2019
-
Abstract
- Adipose-derived stem cells (ADSCs) are a subset of mesenchymal stem cells (MSCs), which have promised a vast therapeutic potential in tissue regeneration. Recent studies have demonstrated that combining stem cells with mechanical stretch may strengthen the efficacy of regenerative therapies. However, the exact influences of mechanical stretch on MSCs still remain inconclusive. In this study, human ADSCs (hADSCs) were applied cyclic stretch stimulation under an in vitro stretching model for designated duration. We found that mechanical stretch significantly promoted the proliferation, adhesion and migration of hADSCs, suppressing cellular apoptosis and increasing the production of pro-healing cytokines. For differentiation of hADSCs, mechanical stretch inhibited adipogenesis, but enhanced osteogenesis. Long-term stretch could promote ageing of hADSCs, but did not alter the cell size and typical immunophenotypic characteristics. Furthermore, we revealed that PI3K/AKT and MAPK pathways might participate in the effects of mechanical stretch on the biological characteristics of hADSCs. Taken together, mechanical stretch is an effective strategy for enhancing stem cell behaviour and regulating stem cell fate. The synergy between hADSCs and mechanical stretch would most likely facilitate tissue regeneration and promote the development of stem cell therapy.<br /> (© 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.)
- Subjects :
- Adipocytes metabolism
Adipogenesis physiology
Adipose Tissue metabolism
Adipose Tissue physiology
Cell Differentiation physiology
Cell Proliferation physiology
Cells, Cultured
Humans
Mesenchymal Stem Cells metabolism
Mesenchymal Stem Cells physiology
Osteogenesis physiology
Phosphatidylinositol 3-Kinases metabolism
Signal Transduction physiology
Stem Cells metabolism
Wound Healing physiology
Adipocytes physiology
Stem Cells physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1582-4934
- Volume :
- 23
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of cellular and molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 31020802
- Full Text :
- https://doi.org/10.1111/jcmm.14314