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Artificial cell membrane binding thrombin constructs drive in situ fibrin hydrogel formation.
- Source :
-
Nature communications [Nat Commun] 2019 Apr 23; Vol. 10 (1), pp. 1887. Date of Electronic Publication: 2019 Apr 23. - Publication Year :
- 2019
-
Abstract
- Cell membrane re-engineering is emerging as a powerful tool for the development of next generation cell therapies, as it allows the user to augment therapeutic cells to provide additional functionalities, such as homing, adhesion or hypoxia resistance. To date, however, there are few examples where the plasma membrane is re-engineered to display active enzymes that promote extracellular matrix protein assembly. Here, we report on a self-contained matrix-forming system where the membrane of human mesenchymal stem cells is modified to display a novel thrombin construct, giving rise to spontaneous fibrin hydrogel nucleation and growth at near human plasma concentrations of fibrinogen. The cell membrane modification process is realised through the synthesis of a membrane-binding supercationic thrombin-polymer surfactant complex. Significantly, the resulting robust cellular fibrin hydrogel constructs can be differentiated down osteogenic and adipogenic lineages, giving rise to self-supporting monoliths that exhibit Young's moduli that reflect their respective extracellular matrix compositions.
- Subjects :
- Animals
Animals, Genetically Modified
Cell Differentiation
Cell Membrane metabolism
Disease Models, Animal
Elastic Modulus
Extracellular Matrix metabolism
Fibroblasts
Humans
Hydrogels chemistry
Hydrogels metabolism
Mesenchymal Stem Cells
Polymers chemistry
Recombinant Proteins chemistry
Recombinant Proteins genetics
Recombinant Proteins metabolism
Surface-Active Agents chemistry
Thrombin genetics
Thrombin metabolism
Zebrafish
Cell Engineering methods
Cell Membrane chemistry
Fibrin metabolism
Thrombin chemistry
Wound Healing
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 10
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 31015421
- Full Text :
- https://doi.org/10.1038/s41467-019-09763-0