Back to Search
Start Over
Green Fluorescent Probe for Imaging His 6 -Tagged Proteins Inside Living Cells.
- Source :
-
ACS sensors [ACS Sens] 2019 May 24; Vol. 4 (5), pp. 1190-1196. Date of Electronic Publication: 2019 May 06. - Publication Year :
- 2019
-
Abstract
- Small molecule-based fluorescent probes offer great opportunities for specifically tracking proteins in living systems with minimal perturbation on the protein function and localization. Herein, we report a small green fluorescent probe (Ni <superscript>2+</superscript> - NTA-AF) consisting of a Ni <superscript>2+</superscript> -NTA moiety, a fluorescein, and an arylazide group, that binds specifically to His <subscript>6</subscript> -tagged proteins with fluorescence enhancement in vitro upon photoactivation of the arylazide group. Importantly, the probe can cross the cell membranes and stoichiometrically label His <subscript>6</subscript> -tagged proteins rapidly (∼15 min) in living prokaryotic and eukaryotic cells exemplified by a DNA repair protein Xeroderma pigmentosum group A (XPA). Using the probe, we successfully visualized Sirtuin 5, which is localized to the mitochondria. This probe exhibits high quantum yields and improved solubility, offering a new opportunity for imaging intracellular His <subscript>6</subscript> -tagged proteins inside living cells with better contrast.
- Subjects :
- Cell Survival
Escherichia coli cytology
Escherichia coli metabolism
HeLa Cells
Humans
Intracellular Space metabolism
Nickel chemistry
Nitrilotriacetic Acid chemistry
Optical Imaging methods
Solubility
Xeroderma Pigmentosum Group A Protein chemistry
Xeroderma Pigmentosum Group A Protein metabolism
Fluorescent Dyes chemistry
Histidine chemistry
Proteins chemistry
Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2379-3694
- Volume :
- 4
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- ACS sensors
- Publication Type :
- Academic Journal
- Accession number :
- 31012309
- Full Text :
- https://doi.org/10.1021/acssensors.8b01128