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Lupeol suppresses migration and invasion via p38/MAPK and PI3K/Akt signaling pathways in human osteosarcoma U-2 OS cells.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2019 Sep; Vol. 83 (9), pp. 1729-1739. Date of Electronic Publication: 2019 Apr 22. - Publication Year :
- 2019
-
Abstract
- Lupeol, one of the common components from the fruits and natural foods, has been reported to exert antitumor activities in many human cancer cell lines; however, its effects on osteosarcoma cell metastasis were not elucidated. In the present study, lupeol at 10-25 μM induced cell morphological changes and decreased total viable cell number in U-2 OS cells. Lupeol (5-15 μM) suppressed cell mobility, migration, and invasion by wound healing and transwell chamber assays, respectively. Lupeol inhibited the activities of MMP-2 and -9 in U-2 OS cells by gelatin zymography assay. Lupeol significantly decreased PI3K, pAKT, β-catenin, and increased GSK3β. Furthermore, lupeol decreased the expressions of Ras, p-Raf-1, p-p38, and β-catenin. Lupeol also decreased uPA, MMP-2, MMP-9, and N-cadherin but increased VE-cadherin in U-2 OS cells. Based on these observations, we suggest that lupeol can be used in anti-metastasis of human osteosarcoma cells in the future.
- Subjects :
- Bone Neoplasms enzymology
Cell Line, Tumor
Cell Survival drug effects
Glycogen Synthase Kinase 3 beta metabolism
Humans
Matrix Metalloproteinases metabolism
Osteosarcoma enzymology
Bone Neoplasms pathology
Mitogen-Activated Protein Kinases metabolism
Neoplasm Invasiveness prevention & control
Neoplasm Metastasis prevention & control
Osteosarcoma pathology
Pentacyclic Triterpenes pharmacology
Phosphatidylinositol 3-Kinases metabolism
Proto-Oncogene Proteins c-akt metabolism
Signal Transduction drug effects
p38 Mitogen-Activated Protein Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 83
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31010399
- Full Text :
- https://doi.org/10.1080/09168451.2019.1606693