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The poly(A) polymerase PAPS1 interacts with the RNA-directed DNA-methylation pathway in sporophyte and pollen development.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2019 Aug; Vol. 99 (4), pp. 655-672. Date of Electronic Publication: 2019 May 30. - Publication Year :
- 2019
-
Abstract
- RNA-based processes play key roles in the regulation of eukaryotic gene expression. This includes both the processing of pre-mRNAs into mature mRNAs ready for translation and RNA-based silencing processes, such as RNA-directed DNA methylation (RdDM). Polyadenylation of pre-mRNAs is one important step in their processing and is carried out by three functionally specialized canonical nuclear poly(A) polymerases in Arabidopsis thaliana. Null mutations in one of these, termed PAPS1, result in a male gametophytic defect. Using a fluorescence-labelling strategy, we have characterized this defect in more detail using RNA and small-RNA sequencing. In addition to global defects in the expression of pollen-differentiation genes, paps1 null-mutant pollen shows a strong overaccumulation of transposable element (TE) transcripts, yet a depletion of 21- and particularly 24-nucleotide-long short interfering RNAs (siRNAs) and microRNAs (miRNAs) targeting the corresponding TEs. Double-mutant analyses support a specific functional interaction between PAPS1 and components of the RdDM pathway, as evident from strong synergistic phenotypes in mutant combinations involving paps1, but not paps2 paps4, mutations. In particular, the double-mutant of paps1 and rna-dependent rna polymerase 6 (rdr6) shows a synergistic developmental phenotype disrupting the formation of the transmitting tract in the female gynoecium. Thus, our findings in A. thaliana uncover a potentially general link between canonical poly(A) polymerases as components of mRNA processing and RdDM, reflecting an analogous interaction in fission yeast.<br /> (© 2019 The Authors The Plant Journal © 2019 John Wiley & Sons Ltd.)
- Subjects :
- Arabidopsis genetics
Arabidopsis metabolism
Arabidopsis Proteins genetics
Arabidopsis Proteins metabolism
DNA Methylation genetics
DNA Methylation physiology
Gene Expression Regulation, Plant genetics
Gene Expression Regulation, Plant physiology
Pollen metabolism
Polynucleotide Adenylyltransferase genetics
RNA, Plant genetics
RNA, Plant metabolism
RNA, Small Interfering genetics
RNA, Small Interfering metabolism
RNA-Dependent RNA Polymerase genetics
RNA-Dependent RNA Polymerase metabolism
Polynucleotide Adenylyltransferase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1365-313X
- Volume :
- 99
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 31009115
- Full Text :
- https://doi.org/10.1111/tpj.14348