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Methylation status and expression patterns of myomaker gene play important roles in postnatal development in the Japanese flounder (Paralichthys olivaceus).
- Source :
-
General and comparative endocrinology [Gen Comp Endocrinol] 2019 Sep 01; Vol. 280, pp. 104-114. Date of Electronic Publication: 2019 Apr 16. - Publication Year :
- 2019
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Abstract
- Myomaker is a membrane protein that plays a crucial role in the fusion of myoblasts during muscle growth. DNA methylation, a significant factor, regulates gene expression. The aim of this study was to examine the methylation and mRNA expression patterns of the myomaker gene during 8 different postnatal developmental stages in the Japanese flounder (L: 7 days post hatch (dph); M1: 21 dph; M2: 28 dph; M3: 35 dph; J1: 90 dph; J2: 180 dph; A1: 24 months; A2: 36 months). Muscle tissue samples were taken from Japanese flounder at different postnatal development stages to measure the extent of DNA methylation and gene expression. Methylation level in the promoter and exon 1 of myomaker was measured using bisulfite sequencing, and the relative expression of myomaker during each developmental stage was measured by quantitative PCR. The relative expression levels of myomaker were up-regulated from stages L to M2, M3 to J2, and methylation of myomaker was negatively correlated with mRNA expression. Furthermore, the CpG site located at -26 bp in the promoter was the lowest methylated region in all developmental stages. These results offer a basis for understanding the mechanism by which myomaker regulates muscle formation during postnatal development.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
CpG Islands genetics
Exons genetics
Japan
Muscle Proteins chemistry
Muscle Proteins metabolism
Muscles drug effects
Muscles metabolism
Phylogeny
Promoter Regions, Genetic
RNA, Messenger genetics
RNA, Messenger metabolism
DNA Methylation genetics
Flounder genetics
Flounder growth & development
Gene Expression Regulation, Developmental
Muscle Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-6840
- Volume :
- 280
- Database :
- MEDLINE
- Journal :
- General and comparative endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 31002826
- Full Text :
- https://doi.org/10.1016/j.ygcen.2019.04.017