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Upregulation of LGMNP1 confers radiotherapy resistance in glioblastoma.
- Source :
-
Oncology reports [Oncol Rep] 2019 Jun; Vol. 41 (6), pp. 3435-3443. Date of Electronic Publication: 2019 Apr 18. - Publication Year :
- 2019
-
Abstract
- Glioblastoma is a lethal brain tumor type, which is frequently resistant to radiotherapy. The aim of the present study was to explore the function of legumain pseudogene 1 (LGMNP1) on radioresistance in glioblastoma. Reverse transcription‑quantitative PCR was used to detect the relative expression of LGMNP1 in glioma cell lines after radiotherapy. Ectopic expression of LGMNP1 was achieved by transfection of a lentiviral vector. A clonogenic assay was used to determine the colony formation ability following radiotherapy. A comet assay, flow cytometry and western blot analysis were applied to detect DNA damage, the apoptotic rate, and levels of apoptotic proteins, respectively. The results revealed that LGMNP1 was significantly upregulated in glioma cells after radiation. Glioma cells stably overexpressing LGMNP1 were successfully established. Overexpression of LGMNP1 in glioma cells reduced DNA damage processes and the percentage of apoptotic cells after radiotherapy. In addition, overexpression of LGMNP1 in glioblastoma multiforme cells decreased apoptotic protein expression after radiotherapy. The present results indicated that upregulation of LGMNP1 conferred radiotherapy resistance by increasing the ability of DNA damage protection and reducing the apoptotic population in glioma cells.
- Subjects :
- Apoptosis radiation effects
Brain Neoplasms genetics
Brain Neoplasms pathology
Cell Line, Tumor
Cell Proliferation radiation effects
DNA Damage radiation effects
DNA Repair radiation effects
Gene Expression Regulation, Neoplastic radiation effects
Glioblastoma genetics
Glioblastoma pathology
Humans
Apoptosis Regulatory Proteins genetics
Brain Neoplasms radiotherapy
Cysteine Endopeptidases genetics
Glioblastoma radiotherapy
Radiation Tolerance genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2431
- Volume :
- 41
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Oncology reports
- Publication Type :
- Academic Journal
- Accession number :
- 31002346
- Full Text :
- https://doi.org/10.3892/or.2019.7128