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Type XI collagen-perlecan-HS interactions stabilise the pericellular matrix of annulus fibrosus cells and chondrocytes providing matrix stabilisation and homeostasis.
- Source :
-
Journal of molecular histology [J Mol Histol] 2019 Jun; Vol. 50 (3), pp. 285-294. Date of Electronic Publication: 2019 Apr 16. - Publication Year :
- 2019
-
Abstract
- The aim of this study was to ascertain whether, like many cell types in cartilaginous tissues if type XI collagen was a pericellular component of annulus fibrosus (AF) cells and chondrocytes. Fine fibrillar networks were visualised which were perlecan, HS (MAb 10E4) and type XI collagen positive. Heparitinase-III pre-digestion abolished the type XI collagen and 10E4 localisation in these fibrillar assemblies demonstrating a putative HS mediated interaction which localised the type XI collagen. Type XI collagen was confirmed to be present in the Heparitinase III treated AF monolayer media samples by immunoblotting. Heparitinase-III generated ΔHS stub epitopes throughout these fibrillar networks strongly visualised by MAb 3-G-10. Monolayers of murine hip articular chondrocytes from C57BL/6 and Hspg2 exon 3 null mice also displayed pericellular perlecan localisations, however type XI collagen was only evident in the Wild type mice. Perlecan was also immunolocalised in control and murine knee articular cartilage from the two mouse genotypes subjected to a medial meniscal destabilisation procedure which induces OA. This resulted in a severe depletion of perlecan levels particularly in the perlecan exon 3 null mice and was consistent with OA representing a disease of the pericellular matrix. A model was prepared to explain these observations between the NPP type XI collagen domain and HS chains of perlecan domain-I in the pericellular matrix of AF cells which likely contributed to cellular communication, tissue stabilization and the regulation of extracellular matrix homeostasis.
- Subjects :
- Animals
Annulus Fibrosus metabolism
Annulus Fibrosus pathology
Cartilage, Articular growth & development
Cartilage, Articular metabolism
Chondrocytes drug effects
Chondrocytes metabolism
Exons genetics
Extracellular Matrix genetics
Extracellular Matrix Proteins genetics
Homeostasis genetics
Intervertebral Disc growth & development
Intervertebral Disc metabolism
Mice
Polysaccharide-Lyases pharmacology
Annulus Fibrosus drug effects
Collagen Type XI genetics
Heparan Sulfate Proteoglycans genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1567-2387
- Volume :
- 50
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of molecular histology
- Publication Type :
- Academic Journal
- Accession number :
- 30993430
- Full Text :
- https://doi.org/10.1007/s10735-019-09823-1