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(-)-Myrtenol accelerates healing of acetic acid-induced gastric ulcers in rats and in human gastric adenocarcinoma cells.
- Source :
-
European journal of pharmacology [Eur J Pharmacol] 2019 Jul 05; Vol. 854, pp. 139-148. Date of Electronic Publication: 2019 Apr 13. - Publication Year :
- 2019
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Abstract
- The gastroprotective property of (-)-myrtenol, a monoterpenoid, has been demonstrated previously against acute gastric ulceration induced by ethanol. However, the healing property of (-)-myrtenol in a chronic gastric ulcer model remains to be verified. This study evaluated its healing efficacy and the mechanism involved using the rat model of chronic gastric ulcer induced by serosal injection of 80% acetic acid in vivo, and human gastric adenocarcinoma cells (AGS) in vitro. The results showed that compared to vehicle-treated ulcer controls, oral administration of (-)-myrtenol (50 and 100 mg/kg/day) for 7 days promoted ulcer healing, as indicated by significant decreases in ulcer area and volume. The macroscopic and microscopic findings confirmed the healing potential of (-)-myrtenol. The ulcer healing activity was also associated with significant increases in gastric mucin content, collagen deposition, number of cells with positive marking for proliferating cell nuclear antigen (PCNA), and by changes in the expression of the inflammatory parameters tumor necrosis factor (TNF)-α, interleukin (IL)-1β and cyclooxygenase (COX)-2, as well as a decrease of metalloproteinases (MMP-9 and MMP-2) activity. Furthermore, in vitro assays using the AGS cultures revealed that (-)-myrtenol favors wound healing activity via stimulation of cell proliferation and migration without altering the cell viability. Taken together, these findings indicate that (-)-myrtenol has gastro-cytoprotective and ulcer healing properties that can be further explored to develop a new therapeutic agent from a natural source for the treatment of gastric ulcer.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cell Line, Tumor
Cell Movement drug effects
Cell Proliferation drug effects
Cell Survival drug effects
Collagen metabolism
Cyclooxygenase 2 genetics
Gene Expression Regulation, Neoplastic drug effects
Glycoproteins metabolism
Humans
Interleukin-1beta genetics
Male
Matrix Metalloproteinase 2 metabolism
Matrix Metalloproteinase 9 metabolism
Proliferating Cell Nuclear Antigen metabolism
RNA, Messenger genetics
RNA, Messenger metabolism
Rats
Rats, Wistar
Stomach drug effects
Stomach pathology
Stomach Ulcer pathology
Tumor Necrosis Factor-alpha genetics
Acetic Acid adverse effects
Adenocarcinoma pathology
Bicyclic Monoterpenes pharmacology
Stomach Neoplasms pathology
Stomach Ulcer physiopathology
Wound Healing drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0712
- Volume :
- 854
- Database :
- MEDLINE
- Journal :
- European journal of pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 30991046
- Full Text :
- https://doi.org/10.1016/j.ejphar.2019.04.025