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Detection of brain specific cardiolipins in plasma after experimental pediatric head injury.
- Source :
-
Experimental neurology [Exp Neurol] 2019 Jun; Vol. 316, pp. 63-73. Date of Electronic Publication: 2019 Apr 11. - Publication Year :
- 2019
-
Abstract
- Cardiolipin (CL) is a mitochondria-specific phospholipid that is central to maintenance and regulation of mitochondrial bioenergetic and metabolic functions. CL molecular species display great tissue variation with brain exhibiting a distinct, highly diverse CL population. We recently showed that the appearance of unique brain-type CLs in plasma could serve as a brain-specific marker of mitochondrial/tissue injury in patients after cardiac arrest. Mitochondrial dysfunction has been increasingly implicated as a critical mechanism underlying the pathogenesis of traumatic brain injury (TBI). Therefore, we hypothesized that unique, brain-specific CL species from the injured brain are released to the peripheral circulation after TBI. To test this hypothesis, we performed a high-resolution mass spectrometry based phospholipidomics analysis of post-natal day (PND)17 rat brain and plasma after controlled cortical impact. We found a time-dependent increase in plasma CLs after TBI including the aforementioned brain-specific CL species early after injury, whereas CLs were significantly decreased in the injured brain. Compositional and quantitative correlational analysis suggested a possible release of CL into the systemic circulation following TBI. The identification of brain-type CLs in systemic circulation may indicate underlying mitochondrial dysfunction/loss after TBI. They may have potential as pharmacodynamics response biomarkers for targeted therapies.<br /> (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Brain Injuries, Traumatic
Cardiolipins blood
Cerebral Cortex injuries
Cerebral Cortex metabolism
Lipidomics
Male
Mass Spectrometry
Mitochondria metabolism
Phospholipids metabolism
Rats
Rats, Sprague-Dawley
Brain Chemistry
Cardiolipins analysis
Cardiolipins metabolism
Craniocerebral Trauma metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2430
- Volume :
- 316
- Database :
- MEDLINE
- Journal :
- Experimental neurology
- Publication Type :
- Academic Journal
- Accession number :
- 30981805
- Full Text :
- https://doi.org/10.1016/j.expneurol.2019.04.007