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MEK1/2 inhibitor U0126, but not nimodipine, reduces upregulation of cerebrovascular contractile receptors after subarachnoid haemorrhage in rats.
- Source :
-
PloS one [PLoS One] 2019 Apr 12; Vol. 14 (4), pp. e0215398. Date of Electronic Publication: 2019 Apr 12 (Print Publication: 2019). - Publication Year :
- 2019
-
Abstract
- Vascular pathophysiological changes after haemorrhagic stroke, such as phenotypic modulation of the cerebral arteries and cerebral vasospasms, are associated with delayed cerebral ischemia (DCI) and poor outcome. The only currently approved drug treatment shown to reduce the risk of DCI and improve neurologic outcome after aneurysmal subarachnoid haemorrhage (SAH) is nimodipine, a dihydropyridine L-type voltage-gated Ca2+ channel blocker. MEK1/2 mediated transcriptional upregulation of contractile receptors, including endothelin-1 (ET-1) receptors, has previously been shown to be a factor in the pathology of SAH. The aim of the study was to compare intrathecal and subcutaneous treatment regimens of nimodipine and intrathecal treatment regimens of U0126, a MEK1/2 inhibitor, in a single injection experimental rat SAH model with post 48 h endpoints consisting of wire myography of cerebral arteries, flow cytometry of cerebral arterial tissue and behavioural evaluation. Following ET-1 concentration-response curves, U0126 exposed arteries had a significantly lower ET-1max than vehicle arteries. Arteries from both the intrathecal- and subcutaneous nimodipine treated animals had significantly higher ET-1max contractions than the U0126 arteries. Furthermore, Ca2+ concentration response curves (precontracted with ET-1 and in the presence of nimodipine) showed that nimodipine treatment could result in larger nimodipine insensitive contractions compared to U0126. Flow cytometry showed decreased protein expression of the ETB receptor in U0126 treated cerebral vascular smooth muscle cells compared to vehicle. Only U0126 treatment lowered ET-1max contractions and ETB receptor levels, as well as decreased the contractions involving nimodipine-insensitive Ca2+ channels, when compared to both intrathecal and subcutaneous nimodipine treatment. This indicate that targeting gene expression might be a better strategy than blocking specific receptors or ion channels in future treatments of SAH.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Animals
Calcium Channel Blockers pharmacology
Cerebral Arteries drug effects
Cerebral Arteries physiopathology
Disease Models, Animal
Male
Mitogen-Activated Protein Kinase Kinases antagonists & inhibitors
Muscle Contraction drug effects
Muscle, Smooth, Vascular drug effects
Muscle, Smooth, Vascular physiopathology
Nimodipine pharmacology
Rats
Rats, Sprague-Dawley
Receptor, Endothelin B genetics
Receptor, Endothelin B physiology
Subarachnoid Hemorrhage genetics
Up-Regulation drug effects
Vasoconstriction drug effects
Vasoconstriction physiology
Butadienes pharmacology
Nitriles pharmacology
Protein Kinase Inhibitors pharmacology
Subarachnoid Hemorrhage drug therapy
Subarachnoid Hemorrhage physiopathology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 14
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 30978262
- Full Text :
- https://doi.org/10.1371/journal.pone.0215398