Back to Search
Start Over
Molecular epidemiology of Leptospira interrogans in Rattus norvegicus in Hanoi, Vietnam.
- Source :
-
Acta tropica [Acta Trop] 2019 Jun; Vol. 194, pp. 204-208. Date of Electronic Publication: 2019 Apr 06. - Publication Year :
- 2019
-
Abstract
- Leptospirosis is a zoonotic disease that is caused by pathogenic spirochaetes of Leptospira spp. and it has become a public health concern in urban localities in the tropics. Rats are important reservoir animals for the transmission of leptospirosis in urban areas. Leptospirosis is considered endemic in Vietnam. However, information on the causative Leptospira genotypes and serotypes in the country is limited. We investigated the carrier status of Leptospira spp. in rats captured in Hanoi by culturing and DNA detection. Isolates were characterized using a serological method and multiple-locus variable-number tandem repeat analysis (MLVA). We captured 144 rats (1 Rattus argentiventer, 135 R. norvegicus, and 8 R. rattus) and obtained 17 L. interrogans, determined by rrs sequencing, from R. norvegicus (12.6%). Sixteen of the isolates were serogroup Bataviae. Five of the 16 isolates exhibited an MLVA type identical to that of the serovar Bataviae reference strain Van Tienen, while there were nine repeats for the other 11 isolates at VNTR31 compared with the reference strain. The remaining isolate grew poorly, and we were unable to determine its serogroup. However, it had an MLVA type matching those of serogroup Pomona strains isolated from R. norvegicus in Japan. Three different flaB sequences were detected in 23 out of 81 R. norvegicus kidney tissue samples (28.4%) using nested PCR followed by DNA sequencing. Two of the sequences were identical with those of serogroups Bataviae and Pomona, and no strain with another sequence was detected in the present study. The present study reveals a high prevalence rate of L. interrogans among R. norvegicus in Hanoi, Vietnam, indicating a potential risk of rat-borne leptospirosis in the area. The present study also demonstrates that a fastidious L. interrogans strain circulates among rats and that molecular detection is crucial in facilitating the accurate determination of reservoir animals.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cities
Genotype
Leptospira isolation & purification
Leptospira interrogans genetics
Leptospirosis epidemiology
Minisatellite Repeats
Polymerase Chain Reaction
Rats
Sequence Analysis, DNA
Serogroup
Vietnam epidemiology
Leptospira interrogans isolation & purification
Leptospirosis veterinary
Molecular Epidemiology
Rodent Diseases epidemiology
Rodent Diseases microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6254
- Volume :
- 194
- Database :
- MEDLINE
- Journal :
- Acta tropica
- Publication Type :
- Academic Journal
- Accession number :
- 30965020
- Full Text :
- https://doi.org/10.1016/j.actatropica.2019.02.008