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AIM2 promotes non-small-cell lung cancer cell growth through inflammasome-dependent pathway.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 2019 Nov; Vol. 234 (11), pp. 20161-20173. Date of Electronic Publication: 2019 Apr 05. - Publication Year :
- 2019
-
Abstract
- The human absent in melanoma 2 (AIM2) is considered as a DNA recognizer. AIM2 has been described as a tumor suppressor gene in the early years. But recent studies suggested that it functions as an oncogene in several cancers. However, its roles in non-small-cell lung cancer (NSCLC) remain unclear. Here we reported that AIM2 highly expressed in NSCLC cells and exhibited a tumor-promoting property both in vitro and in vivo. Besides, AIM2 short hairpin RNA (shRNA)-mediated suppression of cell proliferation was triggered by the accumulation of cells at the G2/M phase. Knockdown of AIM2 reduced the inflammasome formation, while overexpression of AIM2 or stimulation by poly(dA:dT) induced the inflammasome formation. Interestingly, blockade of the inflammasome by caspase-1 inhibitor VX-765 or ASC small interfering RNA (siRNA) abolished the effects brought by AIM2 shRNA and AIM2 plasmid. In summary, our results revealed that AIM2 functioned as an oncogene in NSCLC in an inflammasome-dependent way.<br /> (© 2019 Wiley Periodicals, Inc.)
- Subjects :
- A549 Cells
Animals
Carcinoma, Non-Small-Cell Lung pathology
Caspase 1 genetics
Cell Division genetics
Cell Line, Tumor
G2 Phase genetics
Genes, Tumor Suppressor physiology
Humans
Lung Neoplasms pathology
Mice
Mice, Inbred BALB C
Mice, Nude
RNA, Small Interfering genetics
Carcinoma, Non-Small-Cell Lung genetics
Cell Proliferation genetics
DNA-Binding Proteins genetics
Inflammasomes genetics
Lung Neoplasms genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4652
- Volume :
- 234
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 30953357
- Full Text :
- https://doi.org/10.1002/jcp.28617