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Brefeldin A impairs porcine oocyte meiotic maturation via interruption of organelle dynamics.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 2019 Nov; Vol. 234 (11), pp. 20111-20117. Date of Electronic Publication: 2019 Apr 04. - Publication Year :
- 2019
-
Abstract
- Brefeldin A (BFA) is a lactone antibiotic synthesized from palmitic acid by several fungi that could block anterograde transport of proteins from endoplasmic reticulum to Golgi apparatus by reversible disruption of the Golgi complex. Previous investigations have shown that BFA induces the apoptosis of cancer cells in mitosis and impairs asymmetric spindle positioning in meiosis. Here, we document that exposure to BFA in porcine oocytes compromises the meiotic maturation via disrupting both nuclear and cytoplasmic maturation. We found that BFA exposure collapsed the cytoskeleton assembly by showing the aberrant spindle organization with misaligned chromosomes and defective actin dynamics. Furthermore, the distribution of both mitochondria and cortical granules (CGs), two important indexes of cytoplasmic maturation of oocytes, was disturbed following BFA exposure. We finally validated that the localization of ovastacin, a component of CGs that is essential for the postfertilization removal of sperm-binding sites in the zona pellucida, was also perturbed in BFA-exposed oocytes, which might weaken their fertilization capacity. Collectively, these findings indicate that Golgi-mediated protein transport is indispensable for the porcine oocyte meiotic maturation.<br /> (© 2019 Wiley Periodicals, Inc.)
- Subjects :
- Actins metabolism
Animals
Apoptosis drug effects
Chromosomes drug effects
Cytoplasm drug effects
Cytoplasm metabolism
Cytoskeleton drug effects
Cytoskeleton metabolism
Endoplasmic Reticulum drug effects
Endoplasmic Reticulum metabolism
Female
Fertilization drug effects
Golgi Apparatus drug effects
Golgi Apparatus metabolism
Microtubules drug effects
Microtubules metabolism
Mitochondria drug effects
Mitochondria metabolism
Mitosis drug effects
Oocytes metabolism
Oogenesis drug effects
Swine
Zona Pellucida drug effects
Zona Pellucida metabolism
Brefeldin A pharmacology
Meiosis drug effects
Oocytes drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1097-4652
- Volume :
- 234
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 30950061
- Full Text :
- https://doi.org/10.1002/jcp.28611