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Incomplete cell disruption of resistant microbes.
- Source :
-
Scientific reports [Sci Rep] 2019 Apr 04; Vol. 9 (1), pp. 5618. Date of Electronic Publication: 2019 Apr 04. - Publication Year :
- 2019
-
Abstract
- Biomolecules for OMIC analysis of microbial communities are commonly extracted by bead-beating or ultra-sonication, but both showed varying yields. In addition to that, different disruption pressures are necessary to lyse bacteria and fungi. However, the disruption efficiency and yields comparing bead-beating and ultra-sonication of different biological material have not yet been demonstrated. Here, we show that ultra-sonication in a bath transfers three times more energy than bead-beating over 10 min. TEM imaging revealed intact gram-positive bacterial and fungal cells whereas the gram-negative bacterial cells were destroyed beyond recognition after 10 min of ultra-sonication. DNA extraction using 10 min of bead-beating revealed higher yields for fungi but the extraction efficiency was at least three-fold lower considering its larger genome. By our critical viewpoint, we encourage the review of the commonly used extraction techniques as we provide evidence for a potential underrepresentation of resistant microbes, particularly fungi, in ecological studies.
- Subjects :
- Bacteria genetics
Bacterial Proteins chemistry
DNA chemistry
DNA, Bacterial genetics
Drug Resistance, Bacterial genetics
Drug Resistance, Fungal genetics
Fungal Proteins chemistry
Fungi genetics
Microspheres
Sonication methods
Bacterial Proteins isolation & purification
DNA isolation & purification
Fungal Proteins isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 9
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 30948770
- Full Text :
- https://doi.org/10.1038/s41598-019-42188-9