Evaluation of New 99m Tc(CO) 3 + Radiolabeled Glycylglycine In Vivo .

Background: Peptide-based agents are used in molecular imaging due to their unique properties, such as rapid clearance from the circulation, high affinity and target selectivity. Many of the radiolabeled peptides have been clinically experienced with diagnostic accuracy. The aim of this study was to investigate in vivo biological behavior of [ ^99m Tc(CO) ₃ (H ₂ O) ₃ ]+ radiolabeled glycylglycine (GlyGly).
Methods: Glycylglycine was radiolabeled with a high radiolabeling yield of 94.69±2%, and quality control of the radiolabeling process was performed by thin layer radiochromatography (TLRC) and High-Performance Liquid Radiochromatography (HPLRC). Lipophilicity study for radiolabeled complex ( ^99m Tc(CO) ₃ -Gly-Gly) was carried out using solvent extraction. The in vivo evaluation was performed by both biodistribution and SPECT imaging.
Results: The high radiolabelling yield of ^99m Tc(CO) ₃ -GlyGly was obtained and verified by TLRC and HPLRC as well. According to the in vivo results, SPECT images and biodistribution data are in good accordance. The excretion route from the body was both hepatobiliary and renal.
Conclusion: This study shows that ^99m Tc(CO) ₃ -GlyGly has the potential to be used as a peptide-based imaging agent. Further studies, ^99m Tc(CO) ₃ -GlyGly can be performed on tumor-bearing animals.
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