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The negative effect of magnetic nanoparticles with ascorbic acid on peritoneal macrophages.
- Source :
-
Neurochemical research [Neurochem Res] 2020 Jan; Vol. 45 (1), pp. 159-170. Date of Electronic Publication: 2019 Apr 03. - Publication Year :
- 2020
-
Abstract
- Superparamagnetic iron oxide nanoparticles (SPIOn) are widely used as a contrast agent for cell labeling. Macrophages are the first line of defense of organisms in contact with nanoparticles after their administration. In this study we investigated the effect of silica-coated nanoparticles (γ-Fe <subscript>2</subscript> O <subscript>3</subscript> -SiO <subscript>2</subscript> ) with or without modification by an ascorbic acid (γ-Fe <subscript>2</subscript> O <subscript>3</subscript> -SiO <subscript>2</subscript> -ASA), which is meant to act as an antioxidative agent on rat peritoneal macrophages. Both types of nanoparticles were phagocytosed by macrophages in large amounts as confirmed by transmission electron microscopy and Prusian blue staining, however they did not substantially affect the viability of exposed cells in monitored intervals. We further explored cytotoxic effects related to oxidative stress, which is frequently documented in cells exposed to nanoparticles. Our analysis of double strand breaks (DSBs) marker γH2AX showed an increased number of DSBs in cells treated with nanoparticles. Nanoparticle exposure further revealed only slight changes in the expression of genes involved in oxidative stress response. Lipid peroxidation, another marker of oxidative stress, was not significantly affirmed after nanoparticle exposure. Our data indicate that the effect of both types of nanoparticles on cell viability, or biomolecules such as DNA or lipids, was similar; however the presence of ascorbic acid, either bound to the nanoparticles or added to the cultivation medium, worsened the negative effect of nanoparticles in various tests performed. The attachment of ascorbic acid on the surface of nanoparticles did not have a protective effect against induced cytotoxicity, as expected.
- Subjects :
- Animals
Antioxidants metabolism
Antioxidants toxicity
Cell Survival drug effects
Cell Survival physiology
Cells, Cultured
Dose-Response Relationship, Drug
Drug Synergism
Female
Rats
Rats, Wistar
Ascorbic Acid metabolism
Ascorbic Acid toxicity
Macrophages, Peritoneal drug effects
Macrophages, Peritoneal metabolism
Magnetite Nanoparticles toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6903
- Volume :
- 45
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neurochemical research
- Publication Type :
- Academic Journal
- Accession number :
- 30945145
- Full Text :
- https://doi.org/10.1007/s11064-019-02790-9