Back to Search
Start Over
CpG-DNA induces bacteria-reactive IgM enhancing phagocytic activity against Staphylococcus aureus infection.
- Source :
-
BMB reports [BMB Rep] 2019 Nov; Vol. 52 (11), pp. 635-640. - Publication Year :
- 2019
-
Abstract
- CpG-DNA triggers the proliferation and differentiation of B cells which results in the increased production of antibodies. The presence of bacteria-reactive IgM in normal serum was reported; however, the relevance of CpG-DNA with the production of bacteria-reactive IgM has not been investigated. Here, we proved the function of CpG-DNA for the production of bacteria-reactive IgM. CpG-DNA administration led to increased production of bacteria-reactive IgM both in the peritoneal fluid and serum through TLR9 signaling pathway. When we stimulated B cells with CpG-DNA, production of bacteria-reactive IgM was reproduced in vitro. We established a bacteria-reactive monoclonal IgM antibody using CpG-DNA stimulated-peritoneal B cells. The monoclonal IgM antibody enhanced the phagocytic activity of RAW 264.7 cells against S. aureus MW2 infection. Therefore, we suggest that CpG-DNA enhances the antibacterial activity of the immune system by triggering the production of bacteria-reactive IgM. We also suggest the possible application of the antibodies for the treatment of antibiotics-resistant bacterial infections. [BMB Reports 2019; 52(11): 635-640].
- Subjects :
- Animals
B-Lymphocytes immunology
B-Lymphocytes metabolism
Cell Differentiation drug effects
CpG Islands genetics
DNA, Bacterial
Immunoglobulin M immunology
Mice
Phagocytes
Phagocytosis immunology
RAW 264.7 Cells
Signal Transduction drug effects
Staphylococcus aureus pathogenicity
Toll-Like Receptor 9 genetics
Phagocytosis genetics
Staphylococcal Infections genetics
Staphylococcus aureus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1976-670X
- Volume :
- 52
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- BMB reports
- Publication Type :
- Periodical
- Accession number :
- 30940324