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KEOPS complex promotes homologous recombination via DNA resection.
- Source :
-
Nucleic acids research [Nucleic Acids Res] 2019 Jun 20; Vol. 47 (11), pp. 5684-5697. - Publication Year :
- 2019
-
Abstract
- KEOPS complex is one of the most conserved protein complexes in eukaryotes. It plays important roles in both telomere uncapping and tRNA N6-threonylcarbamoyladenosine (t6A) modification in budding yeast. But whether KEOPS complex plays any roles in DNA repair remains unknown. Here, we show that KEOPS complex plays positive roles in both DNA damage response and homologous recombination-mediated DNA repair independently of its t6A synthesis function. Additionally, KEOPS displays DNA binding activity in vitro, and is recruited to the chromatin at DNA breaks in vivo, suggesting a direct role of KEOPS in DSB repair. Mechanistically, KEOPS complex appears to promote DNA end resection through facilitating the association of Exo1 and Dna2 with DNA breaks. Interestingly, inactivation of both KEOPS and Mre11/Rad50/Xrs2 (MRX) complexes results in synergistic defect in DNA resection, revealing that KEOPS and MRX have some redundant functions in DNA resection. Thus we uncover a t6A-independent role of KEOPS complex in DNA resection, and propose that KEOPS might be a DSB sensor to assist cells in maintaining chromosome stability.<br /> (© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.)
- Subjects :
- Binding, Competitive
Chromatin chemistry
DNA chemistry
DNA Breaks, Double-Stranded
DNA Repair
DNA-Binding Proteins metabolism
Endodeoxyribonucleases metabolism
Exodeoxyribonucleases metabolism
Metalloendopeptidases metabolism
Mutation
Protein Binding
Protein Serine-Threonine Kinases metabolism
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins metabolism
Telomere metabolism
Transcription Factors metabolism
DNA Helicases physiology
DNA, Fungal
Exodeoxyribonucleases physiology
Homologous Recombination
Recombinational DNA Repair
Saccharomyces cerevisiae Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1362-4962
- Volume :
- 47
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Nucleic acids research
- Publication Type :
- Academic Journal
- Accession number :
- 30937455
- Full Text :
- https://doi.org/10.1093/nar/gkz228