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Preparation of Polymerase Chain Reaction Template DNA from Mouse Tail Tissue.
- Source :
-
Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2019 Apr 01; Vol. 2019 (4). Date of Electronic Publication: 2019 Apr 01. - Publication Year :
- 2019
-
Abstract
- A simple cell or tissue lysate can provide a sufficient quality and amount of template DNA for polymerase chain reaction (PCR). In this protocol, a small piece from the tip of the tail is removed and processed using hot sodium hydroxide and Tris (HotSHOT).<br /> (© 2019 Cold Spring Harbor Laboratory Press.)
Details
- Language :
- English
- ISSN :
- 1559-6095
- Volume :
- 2019
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cold Spring Harbor protocols
- Publication Type :
- Academic Journal
- Accession number :
- 30936381
- Full Text :
- https://doi.org/10.1101/pdb.prot092700