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T-cell repertoire profiling by next-generation sequencing reveals tissue migration dynamics of TRBV13-family clonotypes in a common experimental autoimmune encephalomyelitis mouse model.
- Source :
-
Journal of neuroimmunology [J Neuroimmunol] 2019 Jul 15; Vol. 332, pp. 49-56. Date of Electronic Publication: 2019 Mar 23. - Publication Year :
- 2019
-
Abstract
- The experimental autoimmune encephalomyelitis (EAE) model is indispensable for autoimmunity research, but model-specific T cell dynamics are sparsely studied. We used next-generation immunosequencing across lymphoid organs, blood and spinal cord in response to immunization with myelin basic protein (MBP) to study T cell repertoires and migration patterns. Surprisingly, most spinal cord T cells were unique to the individual animal despite the existence of shared MBP-specific clones, suggesting a previously underestimated T cell diversity. Almost complete emigration of pathogenic clones from blood to spinal cord indicates that blood is not a suitable compartment to study EAE-mediating T cells.<br /> (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Autoantigens immunology
Blood Cells immunology
Blood Cells pathology
Cell Movement
Clone Cells immunology
Encephalomyelitis, Autoimmune, Experimental blood
Encephalomyelitis, Autoimmune, Experimental pathology
Female
High-Throughput Nucleotide Sequencing
Lymphoid Tissue immunology
Lymphoid Tissue pathology
Mice
Myelin Basic Protein immunology
Peptide Fragments immunology
Receptors, Antigen, T-Cell, alpha-beta genetics
Specific Pathogen-Free Organisms
Spinal Cord immunology
Spinal Cord pathology
Clonal Selection, Antigen-Mediated genetics
Encephalomyelitis, Autoimmune, Experimental immunology
T-Cell Antigen Receptor Specificity genetics
T-Lymphocyte Subsets immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1872-8421
- Volume :
- 332
- Database :
- MEDLINE
- Journal :
- Journal of neuroimmunology
- Publication Type :
- Academic Journal
- Accession number :
- 30933850
- Full Text :
- https://doi.org/10.1016/j.jneuroim.2019.03.014