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circ-BIRC6, a circular RNA, promotes hepatocellular carcinoma progression by targeting the miR-3918/Bcl2 axis.
- Source :
-
Cell cycle (Georgetown, Tex.) [Cell Cycle] 2019 May; Vol. 18 (9), pp. 976-989. Date of Electronic Publication: 2019 Apr 16. - Publication Year :
- 2019
-
Abstract
- Circular (circ)RNA is a special type of endogenous RNA consisting of a covalently closed loop structure without 5' to 3' polarity and a polyadenylated tail. Accumulating evidence suggests that circRNAs play important roles in the development and progression of human cancers. However, the role of circRNAs in the progression of hepatocellular carcinoma (HCC) is largely unknown. This was addressed in the present study using high-throughput sequencing to identify aberrantly expressed circRNAs in HCC patient tissue and cell lines. We found that circ-baculoviral IAP repeat-containing (BIRC)6 was upregulated in HCC tissue samples and cells; this was associated with the overall survival of HCC patients. circ-BIRC6 knockdown reduced HCC cell proliferation, migration, and invasion and enhanced their apoptosis. Additionally, circ-BIRC6 overexpression negatively regulated the expression of microRNA miR-3918, which was identified as an inhibitor of B cell lymphoma (Bcl)2. The tumor-suppressive effect of circ-BIRC6 deletion was abrogated by inhibiting miR-3918. These results indicate that circ-BIRC6 functions as a competing endogenous RNA that regulates Bcl2 expression by sponging miR-3918, and may serve as a prognostic biomarker and therapeutic target for the treatment of HCC.
- Subjects :
- Animals
Carcinoma, Hepatocellular pathology
Cell Movement genetics
Cell Proliferation genetics
Female
Gene Knockdown Techniques
Hep G2 Cells
Heterografts
Humans
Inhibitor of Apoptosis Proteins genetics
Liver Neoplasms pathology
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Neoplasm Invasiveness genetics
Prognosis
RNA, Circular genetics
Transfection
Tumor Burden genetics
Up-Regulation genetics
Carcinoma, Hepatocellular metabolism
Disease Progression
Inhibitor of Apoptosis Proteins metabolism
Liver Neoplasms metabolism
MicroRNAs metabolism
Proto-Oncogene Proteins c-bcl-2 metabolism
RNA, Circular metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1551-4005
- Volume :
- 18
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Cell cycle (Georgetown, Tex.)
- Publication Type :
- Academic Journal
- Accession number :
- 30931701
- Full Text :
- https://doi.org/10.1080/15384101.2019.1601477