Conservation implications of population genetic structure in a threatened orchid Cypripedium tibeticum .

Cypripedium tibeticum is a threatened orchid which efficient conservation requires knowledge of its extent and structure of genetic variation. Using two chloroplast DNA fragments ( rps 16 and trn L-F), we analyzed 157 individuals from 9 populations representing the species range in China. Seven haplotypes were identified. C. tibeticum had high total genetic diversity ( H _T  = 0.80) with major contribution to this diversity made by among-population component ( G _ST  = 0.64, Φ _ST  = 0.86). However, despite high population differentiation there was no clear phylogeographic structure. The populations CY and DC made the greatest contribution to the total gene diversity as well as allelic richness. The possible mechanisms and implications of these findings for conservation of the species are discussed.