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Label-free detection of Staphylococcus aureus bacteria using long-period fiber gratings with functional polyelectrolyte coatings.
- Source :
-
Biosensors & bioelectronics [Biosens Bioelectron] 2019 May 15; Vol. 133, pp. 147-153. Date of Electronic Publication: 2019 Mar 14. - Publication Year :
- 2019
-
Abstract
- Highly sensitive long-period fiber gratings (LPFG) was developed for label-free and rapid detection of Staphylococcus aureus (S. aureus). Specifically, the LPFG was functionalized with antibody and nanopitted polyelectrolyte coatings to facilitate bacterial adhesion and thus enhance the sensitivity of bacteria detection. The kinetics of S. aureus adhesion on functional coatings were tracked by surface morphology evolution and time-resolved resonance wavelength shift of the coated LPFG at a flow rate of 30 μl/ml and 37 °C in the concentration range of 10 <superscript>4</superscript> -10 <superscript>8</superscript> colony forming unit (CFU)/ml. S. aureus detection at concentrations as low as 224 CFU/ml can be achieved within a short time span of 30 min. The LPFG-based biosensor can be readily adapted to a variety of biophotonic platforms, for applications such as food safety inspection, environmental monitoring, clinical diagnostics, and medical applications.<br /> (Copyright © 2019. Published by Elsevier B.V.)
- Subjects :
- Antibodies chemistry
Humans
Nanocomposites chemistry
Polyelectrolytes chemistry
Staphylococcal Infections microbiology
Staphylococcus aureus pathogenicity
Biosensing Techniques
Staphylococcal Infections diagnosis
Staphylococcus aureus chemistry
Staphylococcus aureus isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4235
- Volume :
- 133
- Database :
- MEDLINE
- Journal :
- Biosensors & bioelectronics
- Publication Type :
- Academic Journal
- Accession number :
- 30927678
- Full Text :
- https://doi.org/10.1016/j.bios.2019.03.024